IMECH-IR  > 力学所知识产出(1956-2008)
Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics
Huang J; Chen J; Chesla SE; Yago T; Mehta P; McEver RP; Zhu C; Long M(龙勉); Zhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA.
Source PublicationJournal of Biological Chemistry
2004
Volume279Issue:43Pages:44915-44923
ISSN0021-9258
AbstractSurface presentation of adhesion receptors influences cell adhesion, although the mechanisms underlying these effects are not well understood. We used a micropipette adhesion frequency assay to quantify how the molecular orientation and length of adhesion receptors on the cell membrane affected two-dimensional kinetic rates of interactions with surface ligands. Interactions of P-selectin, E-selectin, and CD16A with their respective ligands or antibody were used to demonstrate such effects. Randomizing the orientation of the adhesion receptor or lowering its ligand- and antibody-binding domain above the cell membrane lowered two-dimensional affinities of the molecular interactions by reducing the forward rates but not the reverse rates. In contrast, the soluble antibody bound with similar three-dimensional affinities to cell-bound P-selectin constructs regardless of their orientation and length. These results demonstrate that the orientation and length of an adhesion receptor influences its rate of encountering and binding a surface ligand but does not subsequently affect the stability of binding.
Subject Area力学
DOI10.1074/jbc.M407039200
Indexed BySCI
Language英语
WOS IDWOS:000224505600082
WOS KeywordSELECTIN GLYCOPROTEIN LIGAND-1 ; P-SELECTIN ; CELL-ADHESION ; CONCURRENT BINDING ; MEMBRANE ; FLOW ; NEUTROPHILS ; FIBRONECTIN ; AFFINITY ; IDENTIFICATION
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
Citation statistics
Cited Times:96[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/15716
Collection力学所知识产出(1956-2008)
Corresponding AuthorZhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA.
Recommended Citation
GB/T 7714
Huang J,Chen J,Chesla SE,et al. Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics[J]. Journal of Biological Chemistry,2004,279,43,:44915-44923.
APA Huang J.,Chen J.,Chesla SE.,Yago T.,Mehta P.,...&Zhu, C .(2004).Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics.Journal of Biological Chemistry,279(43),44915-44923.
MLA Huang J,et al."Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics".Journal of Biological Chemistry 279.43(2004):44915-44923.
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