IMECH-IR  > 力学所知识产出(1956-2008)
Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions
Long M(龙勉); Zhao H; Huang KS; Zhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA.
Source PublicationAnnals of Biomedical Engineering
2001
Volume29Issue:11Pages:935-946
ISSN0090-6964
AbstractSelectin/ligand interactions initiate the multistep adhesion and signaling cascades in the recruitment of leukocytes from circulation to inflamed tissues and may also play a role in tumor metastasis. Kinetic properties of these interactions are essential determinants governing blood-borne cells' tethering to and rolling on the vessel wall. Extending our recently developed micropipette method, we have measured the kinetic rates of E-selectin/ligand interactions. Red cells coated with an E-selectin construct were allowed to bind HL-60 or Colo-205 cells bearing carbohydrate ligands. Specific adhesions were observed to occur at isolated points, the frequency of which followed a Poisson distribution. These point attachments were formed at the same rate with both the HL-60 and Colo-205 cells (0.14 +/- 0.04 and 0.13 +/- 0.03 mum(2) s(-1) per unit density of E-selectin, respectively) but dissociated from the former at a rate twice as fast as did from the latter (0.92 +/- 0.23 and 0.44 +/- 0.10 s(-1), respectively). The reverse rates agree well with those measured by the flow chamber. The forward rates are orders of magnitude higher than those of Fc gamma receptors interacting with IgG measured under similar conditions, consistent with the rapid kinetics requirement for the function of E-selectin/ligand binding, which is to capture leukocytes on endothelial surfaces from flow.
Subject Area力学
DOI10.1114/1.1415529
Indexed BySCI ; EI
Language英语
WOS IDWOS:000172760500002
WOS KeywordSELECTIN GLYCOPROTEIN LIGAND-1 ; P-SELECTIN ; CARBOHYDRATE INTERACTIONS ; ADHESION MOLECULES ; CONCURRENT BINDING ; HUMAN-LYMPHOCYTES ; HUMAN NEUTROPHILS ; ASSOCIATION RATE ; GROWTH-FACTOR ; CANCER-CELLS
WOS Research AreaEngineering
WOS SubjectEngineering, Biomedical
Citation statistics
Cited Times:59[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/16891
Collection力学所知识产出(1956-2008)
Corresponding AuthorZhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA.
Recommended Citation
GB/T 7714
Long M,Zhao H,Huang KS,et al. Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions[J]. Annals of Biomedical Engineering,2001,29,11,:935-946.
APA Long M,Zhao H,Huang KS,&Zhu, C .(2001).Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions.Annals of Biomedical Engineering,29(11),935-946.
MLA Long M,et al."Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions".Annals of Biomedical Engineering 29.11(2001):935-946.
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