NaCl对液-液扩散法生长溶菌酶晶体的影响

IMECH-IR > 力学所知识产出(1956-2008)

	NaCl对液-液扩散法生长溶菌酶晶体的影响
其他题名	Effect of NaCl on Liquid/Liquid Diffusion Lysozyme Crystal Growth
	戴国亮; 胡文瑞
发表期刊	化学学报
	2003
卷号	61 期号:4 页码:520-525
ISSN	0567-7351
摘要	用动态光散射法研究了不同浓度NaCl对液—液扩散法生长溶菌酶晶体的影响，并测量了晶体生长前后体系的Zeta电势．结果表明，NaCl浓度较高时，在溶菌酶溶液—凝胶界面处会发生液液分层现象，溶液中一直存在较大的聚集体，生长出的晶体质量较差．而在合适的NaCl浓度下，随着溶液Zeta电势降低，溶液中溶菌酶的大的聚集体发生解聚集，生长出的晶体质量较高．
其他摘要	Liquid/liquid diffusion is one of the often-used methods in protein,crystal growth, which is affected by precipitant concentration. The knowledge of. how the precipitant affects the protein crystal growth process is useful to improve the crystal quality. Dynamic light scattering is a powerful tool in studying crystal growth. It is used in this work to reveal the interaction between NaCl and lysozyme molecules/aggregates, which in some degree, can also be described by Zeta potential of protein solution. Concentrations of lysozyme are 15, 25, 35 mg(.)mL(-1), and those of NaCl in agarose are 0.12 kg(.)L(-1) and 0.10 kg(.)L(-1). The results show that there exists liquid/liquid demixing at lysozyme solution-agarose gel interface when NaCl concentration is 0.12 kg(.)L(-1), and some "sea urchin" liked crystals appear. When NaCl concentration decreases to 0.10 kg(.)L(-1), the "sea urchin" liked crystals disappear and the crystals are all tetragonal. From the dynamic. light scattering results, It is believed that the liquid/liquid demixing at the lysozyme solution-agarose gel interface has changed the distribution of NaCl in lysozyme solution by us. So, both existence of large aggregates (larger than 1 muM) of lysozyme and the appearance of "sea urchin" liked crystals are all for lacking of efficient high concentration of NaCl. The Zeta potential of lysozyme solution under several conditions has been measured in our experiments. The Zeta potential decreases at the appropriate crystal growth condition, which reveals that more Cl- are combined with lysozyme molecules. The more combined Cl- is, the smaller the lysozyme aggregates in solution are. That accords with the results of dynamic light scattering.
关键词	液-液扩散法溶菌酶晶体晶体生长动态光散射法 Nacl浓度
学科领域	力学
收录类别	CSCD
语种	中文
WOS记录号	WOS:000187533500012
CSCD记录号	CSCD:1152536
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录] 被引频次：4[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/17131
专题	力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	戴国亮,胡文瑞. NaCl对液-液扩散法生长溶菌酶晶体的影响[J]. 化学学报,2003,61,4,:520-525.
APA	戴国亮,&胡文瑞.(2003).NaCl对液-液扩散法生长溶菌酶晶体的影响.化学学报,61(4),520-525.
MLA	戴国亮,et al."NaCl对液-液扩散法生长溶菌酶晶体的影响".化学学报 61.4(2003):520-525.

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