A Label-Free Protein Microfluidic Array for Parallel Immunoassays

doi:10.1002/elps.200500956

IMECH-IR > 力学所知识产出(1956-2008)

	A Label-Free Protein Microfluidic Array for Parallel Immunoassays
	Wang ZH(王战会); Meng YH(孟永宏); Ying PQ(应佩青); Qi C(齐财); Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China.
发表期刊	Electrophoresis
	2006
卷号	27 期号:20 页码:4078-4085
ISSN	0173-0835
摘要	A label-free protein microfluidic array for immunoassays based on the combination of imaging ellipsometry and an integrated microfluidic system is presented. Proteins can be patterned homogeneously on substrate in array format by the microfluidic system simultaneously. After preparation, the protein array can be packed in the microfluidic system which is full of buffer so that proteins are not exposed to denaturing conditions. With simple microfluidic channel junction, the protein microfluidic array can be used in serial or parallel format to analyze single or multiple samples simultaneously. Imaging ellipsometry is used for the protein array reading with a label-free format. The biological and medical applications of the label-free protein microfluidic array are demonstrated by screening for antibody–antigen interactions, measuring the concentration of the protein solution and detecting five markers of hepatitis B.
学科领域	力学
DOI	10.1002/elps.200500956
收录类别	SCI
语种	英语
WOS记录号	WOS:000241916100020
关键词[WOS]	IMAGING ELLIPSOMETRY ; HIGH-SENSITIVITY ; MICROARRAYS ; SYSTEMS ; ASSAY ; VISUALIZATION ; CHALLENGES ; BIOSENSOR ; SURFACES ; ANTIBODY
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
引用统计	被引频次：57[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/17518
专题	力学所知识产出(1956-2008)
通讯作者	Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China.
推荐引用方式 GB/T 7714	Wang ZH,Meng YH,Ying PQ,et al. A Label-Free Protein Microfluidic Array for Parallel Immunoassays[J]. Electrophoresis,2006,27,20,:4078-4085.
APA	王战会,孟永宏,应佩青,齐财,靳刚,&Jin, G .(2006).A Label-Free Protein Microfluidic Array for Parallel Immunoassays.Electrophoresis,27(20),4078-4085.
MLA	王战会,et al."A Label-Free Protein Microfluidic Array for Parallel Immunoassays".Electrophoresis 27.20(2006):4078-4085.

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