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| Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers | |
Wang ZH(王战会); Viana AS; Jin G(靳刚) ; Abrantes LM; Abrantes, LM (reprint author), Univ Lisbon, Fac Ciencias, Lab SPM, Campo Grande, P-1749016 Lisbon, Portugal.
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| 发表期刊 | Bioelectrochemistry
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| 2006-10 | |
| 卷号 | 69期号:2页码:180-186 |
| ISSN | 1567-5394 |
| 摘要 | An immunosensor interface based on mixed hydrophobic self-assembled monolayers (SAMs) of methyl and carboxylic acid terminated thiols with covalently attached human Immunoglobulin G (hIgG), is investigated. The densely packed and organised SAMs were characterised by contact angle measurements and cyclic voltammetry. The effect of the non-ionic surfactant, Tween 20, in preventing nonspecific adsorption is addressed by ellipsometry during physical and covalent hIgG immobilization on pure and mixed SAMs, respectively. It is clearly demonstrated that nonspecific adsorption due to hydrophobic interactions of hIgG on methyl ended groups is totally inhibited, whereas electrostatic/hydrogen bonding interactions with the exposed carboxylic groups prevail in the presence of surfactant. Results of ellipsometry and Atomic Force Microscopy, reveal that the surface concentration of covalently immobilized hIgG is determined by the ratio of COOH/CH3-terminated thiols in SAM forming solution. Moreover, the ellipsometric data demonstrates that the ratio of bound anti-hIgG/hIgG depends on the density of hIgG on the surface and that the highest ratio is close to three. We also report the selectivity and high sensitivity achieved by chronoamperometry in the detection of adsorbed hIgG and the reaction with its antibody. |
| 关键词 | Mixed Self-assembled Monolayers Immunosensor Human Immunoglobulin g Tween 20 Electrochemistry Ellipsometry |
| 学科领域 | 力学 |
| DOI | 10.1016/j.bioelechem.2006.02.001 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000242005800007 |
| 关键词[WOS] | SURFACE-PLASMON RESONANCE ; SCANNING FORCE MICROSCOPY ; PROTEIN ADSORPTION ; ALKANETHIOLATE MONOLAYERS ; POLY(ETHYLENE GLYCOL) ; REDUCTIVE DESORPTION ; GOLD ELECTRODES ; MODEL SURFACES ; IMMOBILIZATION ; ANTIBODY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Biophysics ; Electrochemistry |
| WOS类目 | Biochemistry & Molecular Biology ; Biology ; Biophysics ; Electrochemistry |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/17676 |
| 专题 | 力学所知识产出(1956-2008) |
| 通讯作者 | Abrantes, LM (reprint author), Univ Lisbon, Fac Ciencias, Lab SPM, Campo Grande, P-1749016 Lisbon, Portugal. |
| 推荐引用方式 GB/T 7714 | Wang ZH,Viana AS,Jin G,et al. Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers[J]. Bioelectrochemistry,2006,69,2,:180-186. |
| APA | Wang ZH,Viana AS,Jin G,Abrantes LM,&Abrantes, LM .(2006).Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers.Bioelectrochemistry,69(2),180-186. |
| MLA | Wang ZH,et al."Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers".Bioelectrochemistry 69.2(2006):180-186. |
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