Measuring Binding Kinetics Of Surface-Bound Molecules Using The Surface Plasmon Resonance Technique

doi:10.1016/j.ab.2008.03.030

IMECH-IR > 力学所知识产出(1956-2008)

	Measuring Binding Kinetics Of Surface-Bound Molecules Using The Surface Plasmon Resonance Technique
	Li BX(李宝霞); Chen J(陈娟); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China.
发表期刊	Analytical Biochemistry
	2008
页码	195-201
ISSN	0003-2697
摘要	Surface plasmon resonance (SPR) technology and the Biacore biosensor have been widely used to measure the kinetics of biomolecular interactions in the fluid phase. In the past decade, the assay was further extended to measure reaction kinetics when two counterpart molecules are anchored on apposed surfaces. However, the cell binding kinetics has not been well quantified. Here we report development of a cellular kinetic model, combined with experimental procedures for cell binding kinetic measurements, to predict kinetic rates per cell. Human red blood cells coated with bovine serum albumin and anti-BSA monoclonal antibodies (mAbs) immobilized on the chip were used to conduct the measurements. Sensor-grams for BSA-coated RBC binding onto and debinding from the anti-BSA mAb-immobilized chip were obtained using a commercial Biacore 3000 biosensor, and analyzed with the cellular kinetic model developed. Not only did the model fit the data well, but it also predicted cellular on and off-rates as well as binding affinities from curve fitting. The dependence of flow duration, flow rate, and site density of BSA on binding kinetics was tested systematically, which further validated the feasibility and reliability of the new approach. Crown copyright (c) 2008 Published by Elsevier Inc. All rights reserved.
关键词	Cellular Off-rate Cellular On-rate Response Unit Kinetic Model Optical Biosensor Literature Selectin Antibody Biacore Antigen Constants Transport Protein Cells Shear
DOI	10.1016/j.ab.2008.03.030
收录类别	SCI
语种	英语
WOS记录号	WOS:000255918000013
关键词[WOS]	OPTICAL BIOSENSOR LITERATURE ; SELECTIN ; ANTIBODY ; BIACORE ; ANTIGEN ; CONSTANTS ; TRANSPORT ; PROTEIN ; CELLS ; SHEAR
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/26038
专题	力学所知识产出(1956-2008)
通讯作者	Long, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China.
推荐引用方式 GB/T 7714	Li BX,Chen J,Long M,et al. Measuring Binding Kinetics Of Surface-Bound Molecules Using The Surface Plasmon Resonance Technique[J]. Analytical Biochemistry,2008:195-201.
APA	李宝霞,陈娟,龙勉,&Long, M .(2008).Measuring Binding Kinetics Of Surface-Bound Molecules Using The Surface Plasmon Resonance Technique.Analytical Biochemistry,195-201.
MLA	李宝霞,et al."Measuring Binding Kinetics Of Surface-Bound Molecules Using The Surface Plasmon Resonance Technique".Analytical Biochemistry (2008):195-201.

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