| Developing A Microfluidic-Based System To Quantify Cell Capture Efficiency |
| Yang F(杨帆); Gao YX(高宇欣); Zhang Y(章燕); Chen J(陈娟); Long M(龙勉); Long M
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发表期刊 | Science in China Series C-Life Sciences
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| 2009
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卷号 | 52期号:2页码:173-181 |
ISSN | 1006-9305
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摘要 | Micro-fabrication technology has substantial potential for identifying molecular markers expressed on the surfaces of tissue cells and viruses. It has been found in several conceptual prototypes that cells with such markers are able to be captured by their antibodies immobilized on microchannel substrates and unbound cells are flushed out by a driven flow. The feasibility and reliability of such a microfluidic-based assay, however, remains to be further tested. In the current work, we developed a microfluidic-based system consisting of a microfluidic chip, an image grabbing unit, data acquisition and analysis software, as well as a supporting base. Specific binding of CD59-expressed or BSA-coupled human red blood cells (RBCs) to anti-CD59 or anti-BSA antibody-immobilized chip surfaces was quantified by capture efficiency and by the fraction of bound cells. Impacts of respective flow rate, cell concentration, antibody concentration and site density were tested systematically. The measured data indicated that the assay was robust. The robustness was further confirmed by capture efficiencies measured from an independent ELISA-based cell binding assay. These results demonstrated that the system developed provided a new platform to effectively quantify cellular surface markers effectively, which promoted the potential applications in both biological studies and clinical diagnoses. |
关键词 | Cell Surface Marker
Microfluidic
Capture Efficiency
Ligand Binding-kinetics
Antibody Microarray
Surface-antigens
Leukemias
Identification
Diagnostics
Membrane
Platform
Design
Marker
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DOI | 10.1007/s11427-009-0017-4
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收录类别 | SCI
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语种 | 英语
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WOS记录号 | WOS:000264106000017
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关键词[WOS] | LIGAND BINDING-KINETICS
; ANTIBODY MICROARRAY
; SURFACE-ANTIGENS
; LEUKEMIAS
; IDENTIFICATION
; DIAGNOSTICS
; MEMBRANE
; PLATFORM
; DESIGN
; MARKER
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WOS研究方向 | Life Sciences & Biomedicine - Other Topics
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WOS类目 | Biology
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引用统计 |
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文献类型 | 期刊论文
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条目标识符 | http://dspace.imech.ac.cn/handle/311007/26718
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专题 | 微重力重点实验室
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通讯作者 | Long M |
推荐引用方式 GB/T 7714 |
Yang F,Gao YX,Zhang Y,et al. Developing A Microfluidic-Based System To Quantify Cell Capture Efficiency[J]. Science in China Series C-Life Sciences,2009,52,2,:173-181.
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APA |
杨帆,高宇欣,章燕,陈娟,龙勉,&Long M.(2009).Developing A Microfluidic-Based System To Quantify Cell Capture Efficiency.Science in China Series C-Life Sciences,52(2),173-181.
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MLA |
杨帆,et al."Developing A Microfluidic-Based System To Quantify Cell Capture Efficiency".Science in China Series C-Life Sciences 52.2(2009):173-181.
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