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Membrane Deformability and Membrane Tension of Single Isolated Mitochondria | |
Wang SQ(王世骐); Jiang CS; Zhang Y(章燕); Chen J(陈娟); Wang B; Long M(龙勉); Long M | |
Source Publication | Cellular And Molecular Bioengineering |
2008 | |
Volume | 1Issue:1Pages:67-74 |
ISSN | 1865-5025 |
Abstract | Mitochondria dynamics is crucial to many biological processes such as mitochondria fusion and fission, which is highly correlated to the mechanics of single mitochondria. However, the mechanobiological coupling of mitochondria has been poorly understood. Here membrane deformability and membrane tension of individual mitochondria isolated from MtDsRed labeled human embryonic T-Rex-293 kidney cells were measured using a micropipette aspiration assay. The results demonstrated that membrane deformation of isolated mitochondria exhibited an elastic transition phase followed by an equilibrium phase, and mitochondrial membrane tension was proportional to the area compressibility. It was also indicated that mitochondrial membrane deformability was significantly affected by physical chemical factors such as osmotic pressure or pH value, and was further correlated to mitochondrial functionality in different respiratory states and Ca2+ regulation. These findings provide a new insight into understanding the mechanical regulation of mitochondrial physiology. |
Keyword | Mechanical Properties Physical-chemical Environment Respiratory State Membrane Permeability Transition Cytochrome-c Oxidative-phosphorylation Permeability Transition Mechanisms Release |
Indexed By | SCI |
Language | 英语 |
WOS ID | WOS:000269821600010 |
Funding Organization | National Natural Science Foundation of China [10332060, 30730032] ; National Key Basic Research Foundation of China [2006CB910303] ; Chinese Academy of Sciences [2005-1-16] |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://dspace.imech.ac.cn/handle/311007/33565 |
Collection | 力学所知识产出(1956-2008) |
Corresponding Author | Long M |
Recommended Citation GB/T 7714 | Wang SQ,Jiang CS,Zhang Y,et al. Membrane Deformability and Membrane Tension of Single Isolated Mitochondria[J]. Cellular And Molecular Bioengineering,2008,1,1,:67-74. |
APA | Wang SQ.,Jiang CS.,Zhang Y.,Chen J.,Wang B.,...&Long M.(2008).Membrane Deformability and Membrane Tension of Single Isolated Mitochondria.Cellular And Molecular Bioengineering,1(1),67-74. |
MLA | Wang SQ,et al."Membrane Deformability and Membrane Tension of Single Isolated Mitochondria".Cellular And Molecular Bioengineering 1.1(2008):67-74. |
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