IMECH-IR  > 力学所知识产出(1956-2008)
Membrane Deformability and Membrane Tension of Single Isolated Mitochondria
Wang SQ(王世骐); Jiang CS; Zhang Y(章燕); Chen J(陈娟); Wang B; Long M(龙勉); Long M
Source PublicationCellular And Molecular Bioengineering
2008
Volume1Issue:1Pages:67-74
ISSN1865-5025
Abstract

Mitochondria dynamics is crucial to many biological processes such as mitochondria fusion and fission, which is highly correlated to the mechanics of single mitochondria. However, the mechanobiological coupling of mitochondria has been poorly understood. Here membrane deformability and membrane tension of individual mitochondria isolated from MtDsRed labeled human embryonic T-Rex-293 kidney cells were measured using a micropipette aspiration assay. The results demonstrated that membrane deformation of isolated mitochondria exhibited an elastic transition phase followed by an equilibrium phase, and mitochondrial membrane tension was proportional to the area compressibility. It was also indicated that mitochondrial membrane deformability was significantly affected by physical chemical factors such as osmotic pressure or pH value, and was further correlated to mitochondrial functionality in different respiratory states and Ca2+ regulation. These findings provide a new insight into understanding the mechanical regulation of mitochondrial physiology.

KeywordMechanical Properties Physical-chemical Environment Respiratory State Membrane Permeability Transition Cytochrome-c Oxidative-phosphorylation Permeability Transition Mechanisms Release
Indexed BySCI
Language英语
WOS IDWOS:000269821600010
Funding OrganizationNational Natural Science Foundation of China [10332060, 30730032] ; National Key Basic Research Foundation of China [2006CB910303] ; Chinese Academy of Sciences [2005-1-16]
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Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/33565
Collection力学所知识产出(1956-2008)
Corresponding AuthorLong M
Recommended Citation
GB/T 7714
Wang SQ,Jiang CS,Zhang Y,et al. Membrane Deformability and Membrane Tension of Single Isolated Mitochondria[J]. Cellular And Molecular Bioengineering,2008,1,1,:67-74.
APA Wang SQ.,Jiang CS.,Zhang Y.,Chen J.,Wang B.,...&Long M.(2008).Membrane Deformability and Membrane Tension of Single Isolated Mitochondria.Cellular And Molecular Bioengineering,1(1),67-74.
MLA Wang SQ,et al."Membrane Deformability and Membrane Tension of Single Isolated Mitochondria".Cellular And Molecular Bioengineering 1.1(2008):67-74.
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