IMECH-IR  > 力学所知识产出(1956-2008)
Impact of carrier stiffness and microtopology on two-dimensional kinetics of P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) interactions
Wu L; Xiao BT; Jia XL(贾潇凌); Zhang Y(章燕); Lv SQ(吕守芹); Chen J(陈娟); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
Source PublicationJournal of Biological Chemistry
2007
Volume282Issue:13Pages:9846-9854
ISSN0021-9258
AbstractMechanics and surface microtopology of the molecular carrier influence cell adhesion, but the mechanisms underlying these effects are not well understood. We used a micropipette adhesion frequency assay to quantify how the carrier stiffness and microtopology affected two-dimensional kinetics of interacting adhesion molecules on two apposing surfaces. Interactions of P-selectin with P-selectin glycoprotein ligand-1 (PSGL-1) were used to demonstrate such effects by presenting the molecules on three carrier systems: human red blood cells (RBCs), human promyelocytic leukemia HL-60 cells, and polystyrene beads. Stiffening the carrier alone or in cooperation with roughing the surface lowered the two-dimensional affinity of interacting molecules by reducing the forward rate but not the reverse rate, whereas softening the carrier and roughing the surface had opposing effects in affecting two-dimensional kinetics. In contrast, the soluble antibody bound with similar three-dimensional affinity to surface-anchored P-selectin or PSGL-1 constructs regardless of carrier stiffness and microtopology. These results demonstrate that the carrier stiffness and microtopology of a receptor influences its rate of encountering and binding a surface ligand but does not subsequently affect the stability of binding. This provides new insights into understanding the rolling and tethering mechanism of leukocytes onto endothelium in both physiological and pathological processes.
KeywordHuman Neutrophils Binding-kinetics Adhesion Affinity Rates Flow
DOI10.1074/jbc.M609219200
Indexed BySCI
Language英语
WOS IDWOS:000245421700059
WOS KeywordHUMAN NEUTROPHILS ; BINDING-KINETICS ; ADHESION ; AFFINITY ; RATES ; FLOW
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
Citation statistics
Cited Times:50[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/34011
Collection力学所知识产出(1956-2008)
Corresponding AuthorLong, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
Recommended Citation
GB/T 7714
Wu L,Xiao BT,Jia XL,et al. Impact of carrier stiffness and microtopology on two-dimensional kinetics of P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) interactions[J]. Journal of Biological Chemistry,2007,282,13,:9846-9854.
APA Wu L.,Xiao BT.,贾潇凌.,章燕.,吕守芹.,...&Long, M .(2007).Impact of carrier stiffness and microtopology on two-dimensional kinetics of P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) interactions.Journal of Biological Chemistry,282(13),9846-9854.
MLA Wu L,et al."Impact of carrier stiffness and microtopology on two-dimensional kinetics of P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) interactions".Journal of Biological Chemistry 282.13(2007):9846-9854.
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