气相扩散法生长溶菌酶晶体的动态光散射研究

doi:10.3866/PKU.WHXB20010612

IMECH-IR > 力学所知识产出(1956-2008)

	气相扩散法生长溶菌酶晶体的动态光散射研究
	戴国亮; 董向群; 孙祉伟; 胡文瑞
发表期刊	物理化学学报=Acta Physico-Chimica Sinica
	2001-06-30
卷号	17 期号:6 页码:531-536
ISSN	1000-6818
摘要	首次采用动态光散射研究了气相扩散法生长溶菌酶晶体 .实验中采用了两种溶解溶菌酶的方法，所得实验结果是有区别的 .这种区别表明了 NaCl对溶菌酶分子间相互作用产生十分重要的影响 .实验结果表明，晶体生长过程中，溶液中溶菌酶始终保持单分子与两分子聚集体的状态，这种状态是生长晶体的基础 .
其他摘要	Both vapor diffusion approach and batch mode are often used approaches for protein crystallization. The light scattering method is a powerful tool particularly for prenucleation study. However, all light scattering studies were limited to the crystallization process of the batch mode. To apply dynamic light scattering technique to the protein crystallization study for the vapor diffusion approach, as well as the batch mode, a special sample cell (see Fig. 1) was desired in the present work. It has two separate parts, a scattering cell and a thin tube filled with salt solution. The results showed that our special cell was reliable for light scattering study(see table 1). In our experiments, vapor diffusion approach was mainly used. Sodium acetate buffer solution, 0.04 mol . L-1 pH = 4.5, was mixed with sodium chloride, whose final concentration was 0.345 mol L-1. Chicken egg white lysozyme was dissolved in the above solution with a concentration of 50 mg . mL(-1) and 25 mg . mL(-1). Two lysozyme dissolution ways were used. One was lysozyme dissolved in only sodium acetate buffer solution and another is in buffer solution with sodium chloride. Their results were different(see Fig. 2 and Fig. 5), that showed NaCl played an important role in the interaction of lysozyme molecules. Due to the feature of vapor diffusion approach, it was verified that the force between lysozyme moleculars in the solution is weak during the whole crystallization process, which is desirable for crystal growth, namely, lysozyme molecules kept in monomolecule or aggregation of bimolecules in solution when lysozyme crystal grew. Besides, our results show that there is a significant difference between this approach and the batch mode(decreasing temperature) even if other parameters are the same. This significant difference is the direct result of different ways of supersaturation, Vapor diffusion approach, compared to batch mode is a more temperate, precisely controlled way to realize supersaturation.
关键词	动态光散射批量法气相扩散法 Dynamic Light Scatter 晶体生长 Vapor Diffusion Approach 溶菌酶 Crystal Growth 扩散系数 Lysozyme Diffusion Coefficient Batch Method
DOI	10.3866/PKU.WHXB20010612
收录类别	SCI ; CSCD
语种	中文
WOS记录号	WOS:000169596200012
关键词[WOS]	CRYSTALLIZATION; NUCLEATION; PROTEINS
CSCD记录号	CSCD:781894
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/40934
专题	力学所知识产出(1956-2008)
通讯作者	戴国亮
推荐引用方式 GB/T 7714	戴国亮,董向群,孙祉伟,等. 气相扩散法生长溶菌酶晶体的动态光散射研究[J]. 物理化学学报=Acta Physico-Chimica Sinica,2001,17,6,:531-536.
APA	戴国亮,董向群,孙祉伟,&胡文瑞.(2001).气相扩散法生长溶菌酶晶体的动态光散射研究.物理化学学报=Acta Physico-Chimica Sinica,17(6),531-536.
MLA	戴国亮,et al."气相扩散法生长溶菌酶晶体的动态光散射研究".物理化学学报=Acta Physico-Chimica Sinica 17.6(2001):531-536.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
471.pdf（338KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载