IMECH-IR  > 力学所知识产出(1956-2008)
Alternative TitleThe Effect of Vapor Diffusion Rate on Lysozyme Crystal Growth
戴国亮; 董向群; 孙祉伟; 胡文瑞
Source Publication物理化学学报=Acta Physico-Chimica Sinica
Other AbstractA sample cell was specially designed and improved for studying crystal growth of lysozyme using vapor diffusion approach by dynamic light scattering. Various rates of vapor diffusion can be easily achieved by adjusting the concentration of the precipitator. A series of experiments using 1, 3 and 4 mol . L-1 NaCl solution and silica gel particles as precipitator were performed and lead us to the conclusion that the rate of vapor diffusion was of great importance for controlling crystal growth. For higher precipitator concentrations (3, 4 mol . L-1 NaCl solutions and silica gel particles), the sizes of particles in the initial stage fluctuate very considerably, while it was not the case for lower concentrations (1 mol . L-1 NaCl solution). During the nucleation stage, the time period decreased with the increasing concentration of the precipitator, but the diameters of particles remained at about 4 similar to 6 nm in despite of different rates of vapor diffusion. This shows that the rate of forming supersaturation plays an important role in crystal growth process.
Keyword气相扩散 样品池 溶菌酶 Vapor Diffusion 晶体生长 Lysozyme 动态光散射 Crystal Growth Dynamic Light Scattering Sample Cell
Indexed BySCI ; CSCD
WOS IDWOS:000173367900016
Funding Organization国家自然科学基金(19789201)
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding Author戴国亮
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GB/T 7714
戴国亮,董向群,孙祉伟,等. 气相扩散速率对溶菌酶晶体生长的影响[J]. 物理化学学报=Acta Physico-Chimica Sinica,2002,18(1):70-73.
APA 戴国亮,董向群,孙祉伟,&胡文瑞.(2002).气相扩散速率对溶菌酶晶体生长的影响.物理化学学报=Acta Physico-Chimica Sinica,18(1),70-73.
MLA 戴国亮,et al."气相扩散速率对溶菌酶晶体生长的影响".物理化学学报=Acta Physico-Chimica Sinica 18.1(2002):70-73.
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