IMECH-IR  > 力学所知识产出(1956-2008)
肝癌细胞与内皮细胞的粘附力学特性研究
Alternative TitleStudies on the adhesive mechanical roperties between hepatocellular carcinoma cells and endothelial cells
宋关斌; 刘保安; 秦建; 龙勉; 蔡绍皙
Source Publication生物物理学报
2003-03-30
Volume19Issue:1Pages:84-87
Abstract采用细胞同步技术和微管吸吮技术,从细胞周期的角度研究不同细胞周期肝癌细胞(hepatocellular carcinoma cells,HCC)与脐静脉内皮细胞(HUVEC)的粘附力学特性。结果表明,未同步化肝癌细胞各周期时相的细胞百分比为:G0/G1期,53.51;G2/M期,11.01;S期,35.48。采用胸腺嘧啶脱氧核苷、秋水仙碱顺序阻断和胸腺嘧啶脱氧核苷双阻断后释放培养的方法可分别获得G1期和S期的肝癌细胞,其平均同步率分别为69.02和96.50。G1期肝癌细胞与人脐静脉内皮细胞的粘附力比S期相应值明显降低(P<0.01),与未同步化肝癌细胞组比较也得到同样结果,而S期与未同步化肝癌细胞组的粘附力值无明显差别。肝癌细胞与脐静脉内皮细胞的粘附力随着附时间的变化而变化,在30-60min内迅速增长,60min之后维持在较稳定的水平,即300×10~-10N左右。提示:在肝癌细胞与内皮细胞的粘附过程中,S期细胞可能起的作用更大;肝癌细胞和内皮细胞上粘附分子表达呈现时间效应,从而体现出粘附和去粘附的行为特征。
Keyword肝癌细胞 内皮细胞 粘附 细胞周期 微管吸吮
Indexed ByCSCD
Language中文
Funding Organization国家自然科学基金项目(19972077)
CSCD IDCSCD:1349254
Citation statistics
Cited Times:3[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/42324
Collection力学所知识产出(1956-2008)
Corresponding Author宋关斌
Recommended Citation
GB/T 7714
宋关斌,刘保安,秦建,等. 肝癌细胞与内皮细胞的粘附力学特性研究[J]. 生物物理学报,2003,19(1):84-87.
APA 宋关斌,刘保安,秦建,龙勉,&蔡绍皙.(2003).肝癌细胞与内皮细胞的粘附力学特性研究.生物物理学报,19(1),84-87.
MLA 宋关斌,et al."肝癌细胞与内皮细胞的粘附力学特性研究".生物物理学报 19.1(2003):84-87.
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