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An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation
Huo B(霍波); Lu XL; Costa KD; Xu QB; Guo XE
Source PublicationCell Calcium
2010
Volume47Issue:3Pages:234-241
ISSN0143-4160
AbstractTo investigate the roles of intercellular gap junctions and extracellular ATP diffusion in bone cell calcium signaling propagation in bone tissue, in vitro bone cell networks were constructed by using microcontact printing and self-assembled monolayer technologies. In the network, neighboring cells were interconnected through functional gap junctions. A single cell at the center of the network was mechanically stimulated by using an AFM nanoindenter. Intracellular calcium ([Ca2+](i)) responses of the bone cell network were recorded and analyzed. In the untreated groups, calcium propagation from the stimulated cell to neighboring cells was observed in 40% of the tests. No significant difference was observed in this percentage when the intercellular gap junctions were blocked. This number, however, decreased to 10% in the extracellular ATP-pathway-blocked group. When both the gap junction and ATP pathways were blocked, intercellular calcium waves were abolished. When the intracellular calcium store in ER was depleted, the indented cell can generate calcium transients, but no [Ca2+](i) signal can be propagated to the neighboring cells. No [Ca2+](i) response was detected in the cell network when the extracellular calcium source was removed. These findings identified the biochemical pathways involved in the calcium signaling propagation in bone cell networks. Published by Elsevier Ltd.
DOI10.1016/j.ceca.2009.12.005
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Indexed BySCI
Language英语
WOS IDWOS:000276430100004
WOS KeywordOSTEOBLASTIC CELLS ; INOSITOL TRISPHOSPHATE ; RECEPTOR ACTIVATION ; EXTRACELLULAR ATP ; EPITHELIAL-CELLS ; GAP-JUNCTIONS ; CA2+ SIGNALS ; PROPAGATION ; WAVES ; COMMUNICATION
WOS Research AreaCell Biology
WOS SubjectCell Biology
Funding OrganizationNIH [R21 AR052417]
Citation statistics
Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/43669
Collection国家微重力实验室
Corresponding AuthorGuo XE
Recommended Citation
GB/T 7714
Huo B,Lu XL,Costa KD,et al. An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation[J]. Cell Calcium,2010,47(3):234-241.
APA 霍波,Lu XL,Costa KD,Xu QB,&Guo XE.(2010).An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation.Cell Calcium,47(3),234-241.
MLA 霍波,et al."An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation".Cell Calcium 47.3(2010):234-241.
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