| Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry |
| Qi C(齐财); Tian XS ; Chen S(陈涉); Yan JH; Cao Z; Tian KG; Gao GF; Jin G(靳刚); Jin G
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| 发表期刊 | Biosensors & Bioelectronics
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| 2010
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| 卷号 | 25期号:6页码:1530-1534 |
| ISSN | 0956-5663
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| 摘要 | A novel method is reported for the detection of avian influenza virus subtype H5 using a biosensor based on high spatial resolution imaging ellipsometry (IE). Monoclonal antibodies specific to H5 hemagglutinin protein were immobilized on silicon wafers and used to capture virus particles. Resultant changes on the surface of the wafers were visualized directly in gray-scale on an imaging ellipsometry image. This preliminary study has shown that the assay is rapid and specific for the identification of avian influenza virus subtype H5. Compared with lateral-flow immunoassays, this biosensor not only has better sensitivity, but can also simultaneously perform multiplexed tests. These results suggest that this biosensor might be a valuable diagnostic toot for avian influenza virus detection. (c) 2009 Elsevier B.V. All rights reserved. |
| DOI | 10.1016/j.bios.2009.10.030
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| URL | 查看原文
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| 收录类别 | SCI
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| 语种 | 英语
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| WOS记录号 | WOS:000274868800045
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| 关键词[WOS] | OFF-NULL ELLIPSOMETRY
; PROTEIN
; SURFACE
; IMMOBILIZATION
; INTERFACES
; BINDING
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| WOS研究方向 | Biophysics
; Biotechnology & Applied Microbiology
; Chemistry
; Electrochemistry
; Science & Technology - Other Topics
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| WOS类目 | Biophysics
; Biotechnology & Applied Microbiology
; Chemistry, Analytical
; Electrochemistry
; Nanoscience & Nanotechnology
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| 项目资助者 | National Basic Research Program of China [2009CB320300]
; National High Technology Research and Development Program (863) of China [2006AA02Z4C2]
; National Basic Research Program (863) of China [2006AA02Z4C2]
; National Basic Research Program (Project 973) of China [2007CB310505]
; National Key Technologies RD Program [2006BAD06A01]
; Chinese Academy of Sciences [KJCX2-YW-Mo3, -M04]
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/43677
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| 专题 | 微重力重点实验室
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| 通讯作者 | Jin G |
推荐引用方式
GB/T 7714 |
Qi C,Tian XS,Chen S,et al. Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry[J]. Biosensors & Bioelectronics,2010,25,6,:1530-1534.
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| APA |
齐财.,Tian XS.,陈涉.,Yan JH.,Cao Z.,...&Jin G.(2010).Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry.Biosensors & Bioelectronics,25(6),1530-1534.
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| MLA |
齐财,et al."Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry".Biosensors & Bioelectronics 25.6(2010):1530-1534.
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