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Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry
Qi C(齐财); Tian XS; Chen S(陈涉); Yan JH; Cao Z; Tian KG; Gao GF; Jin G(靳刚); Jin G
Source PublicationBiosensors & Bioelectronics
2010
Volume25Issue:6Pages:1530-1534
ISSN0956-5663
AbstractA novel method is reported for the detection of avian influenza virus subtype H5 using a biosensor based on high spatial resolution imaging ellipsometry (IE). Monoclonal antibodies specific to H5 hemagglutinin protein were immobilized on silicon wafers and used to capture virus particles. Resultant changes on the surface of the wafers were visualized directly in gray-scale on an imaging ellipsometry image. This preliminary study has shown that the assay is rapid and specific for the identification of avian influenza virus subtype H5. Compared with lateral-flow immunoassays, this biosensor not only has better sensitivity, but can also simultaneously perform multiplexed tests. These results suggest that this biosensor might be a valuable diagnostic toot for avian influenza virus detection. (c) 2009 Elsevier B.V. All rights reserved.
DOI10.1016/j.bios.2009.10.030
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Indexed BySCI
Language英语
WOS IDWOS:000274868800045
WOS KeywordOFF-NULL ELLIPSOMETRY ; PROTEIN ; SURFACE ; IMMOBILIZATION ; INTERFACES ; BINDING
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
Funding OrganizationNational Basic Research Program of China [2009CB320300] ; National High Technology Research and Development Program (863) of China [2006AA02Z4C2] ; National Basic Research Program (863) of China [2006AA02Z4C2] ; National Basic Research Program (Project 973) of China [2007CB310505] ; National Key Technologies RD Program [2006BAD06A01] ; Chinese Academy of Sciences [KJCX2-YW-Mo3, -M04]
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Cited Times:41[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/43677
Collection国家微重力实验室
Corresponding AuthorJin G
Recommended Citation
GB/T 7714
Qi C,Tian XS,Chen S,et al. Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry[J]. Biosensors & Bioelectronics,2010,25(6):1530-1534.
APA 齐财.,Tian XS.,陈涉.,Yan JH.,Cao Z.,...&Jin G.(2010).Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry.Biosensors & Bioelectronics,25(6),1530-1534.
MLA 齐财,et al."Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry".Biosensors & Bioelectronics 25.6(2010):1530-1534.
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