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Intercellular calcium wave propagation in linear and circuit-like bone cell networks
Huo B(霍波); Lu XL; Guo XE
Source PublicationPhilosophical Transactions of The Royal Society A-Mathematical Physical and Engineering Sciences
2010
Volume368Issue:1912Pages:617-633
ISSN1364-503X
AbstractIn the present study, the mechanism of intercellular calcium wave propagation in bone cell networks was identified. By using micro-contact printing and self-assembled monolayer technologies, two types of in vitro bone cell networks were constructed: open-ended linear chains and looped hexagonal networks with precisely controlled intercellular distances. Intracellular calcium responses of the cells were recorded and analysed when a single cell in the network was mechanically stimulated by nano-indentation. The looped cell network was shown to be more efficient than the linear pattern in transferring calcium signals from cell to cell. This phenomenon was further examined by pathway-inhibition studies. Intercellular calcium wave propagation was significantly impeded when extracellular adenosine triphosphate (ATP) in the medium was hydrolysed. Chemical uncoupling of gap junctions, however, did not significantly decrease the transferred distance of the calcium wave in the cell networks. Thus, it is extracellular ATP diffusion, rather than molecular transport through gap junctions, that dominantly mediates the transmission of mechanically elicited intercellular calcium waves in bone cells. The inhibition studies also demonstrated that the mechanical stimulation-induced calcium responses required extracellular calcium influx, whereas the ATP-elicited calcium wave relied on calcium release from the calcium store of the endoplasmic reticulum.
DOI10.1098/rsta.2009.0221
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000273233400006
WOS KeywordFLUID-FLOW ; OSTEOBLASTIC CELLS ; GAP-JUNCTIONS ; ATP ; MECHANOTRANSDUCTION ; COMMUNICATION ; OSCILLATIONS ; PERIODS ; ACID
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
Funding OrganizationNIH [R21 AR052417]
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/43687
Collection国家微重力实验室
Corresponding AuthorGuo XE
Recommended Citation
GB/T 7714
Huo B,Lu XL,Guo XE. Intercellular calcium wave propagation in linear and circuit-like bone cell networks[J]. Philosophical Transactions of The Royal Society A-Mathematical Physical and Engineering Sciences,2010,368(1912):617-633.
APA 霍波,Lu XL,&Guo XE.(2010).Intercellular calcium wave propagation in linear and circuit-like bone cell networks.Philosophical Transactions of The Royal Society A-Mathematical Physical and Engineering Sciences,368(1912),617-633.
MLA 霍波,et al."Intercellular calcium wave propagation in linear and circuit-like bone cell networks".Philosophical Transactions of The Royal Society A-Mathematical Physical and Engineering Sciences 368.1912(2010):617-633.
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