PEGylated Phospholipid Membrane on Polymer Cushion and Its Interaction with Cholesterol

doi:10.1021/la100973p

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	PEGylated Phospholipid Membrane on Polymer Cushion and Its Interaction with Cholesterol
	Zhang YB; Chen YY(陈艳艳); Jin G(靳刚)
发表期刊	Langmuir
	2010
卷号	26 期号:13 页码:11140-11144
ISSN	0743-7463
摘要	By employing poly(ethylene glycol) (PEG) shielding and a polymer cushion to achieve air stability of the lipid membrane, we have analyzed PEG influence on dried membranes and the interaction with cholesterol. Small unilamellar vesicles (SUVs) formed by the mixture of 1,2-dimyristoylphosphatidylcholine (DMPC) with different molar fraction of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(poly(ethylene glycol))-2000] (DSPE-PEG(2000)) adsorb and fuse into membranes on different polymer-modified silicon dioxide surfaces, including chitosan, poly(L-lysine) (PLL), and hyaluronic acid, Dried membranes arc further examined by ellipsometer and atomic force microscopy (AFM). Only chitosan can support a visible and uniform lipid array. The thickness of dry PEGylated lipid membrane is reduced gradually as the molar fraction of PEG increases. AFM scanning confirms the lipid membrane stacking for vesicles containing low PEG, and only a proper amount of PEG can maintain a single lipid hi lover; however, the air stability of the membrane will be destroyed if overloading. PEG. Cholesterol incorporation can greatly improve the structural stability of lipid membrane, especially for those containing high molar fraction of PEG. Different amounts of cholesterol influence the thickness and surface morphology of dried membrane.
DOI	10.1021/la100973p
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000279239900099
关键词[WOS]	CONDENSED COMPLEXES ; IMAGING ELLIPSOMETRY ; BILAYER-MEMBRANES ; MICROARRAYS ; MONOLAYER ; STABILITY ; RESONANCE ; SYSTEM ; LIPIDS ; FLUID
WOS研究方向	Chemistry ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary
项目资助者	Ministry of Science and Technology of China [2008AA02Z419] ; Natural Science Foundation of China [10804083, 20845003] ; Chinese Academy of Sciences [KJCX2. YW.M04] ; Key Laboratory of Microgravity, Institute of Mechanics, CAS
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/43695
专题	微重力重点实验室
推荐引用方式 GB/T 7714	Zhang YB,Chen YY,Jin G. PEGylated Phospholipid Membrane on Polymer Cushion and Its Interaction with Cholesterol[J]. Langmuir,2010,26,13,:11140-11144.
APA	Zhang YB,陈艳艳,&靳刚.(2010).PEGylated Phospholipid Membrane on Polymer Cushion and Its Interaction with Cholesterol.Langmuir,26(13),11140-11144.
MLA	Zhang YB,et al."PEGylated Phospholipid Membrane on Polymer Cushion and Its Interaction with Cholesterol".Langmuir 26.13(2010):11140-11144.

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