| Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching |
| Zhong Y; Kong D(孔冬) ; Dai LH(戴兰宏); Ji BH(季葆华); Ji, BH (reprint author), Beijing Inst Technol, Biomech & Biomat Lab, Dept Appl Mech, Beijing 100081, Peoples R China
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| 发表期刊 | Cellular and Molecular Bioengineering
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| 2011
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| 卷号 | 4期号:3页码:442-456 |
| ISSN | 1865-5025
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| 摘要 | The adhesion-based cell mechanosensitivity plays central roles in many physiological and pathological processes. Recently, quantitative understanding of cell responses to external force has been intensively pursued. However, the frequency dependent cell responses to the substrate stretching have not yet been fully understood. Here we developed a multiscale modeling framework for studying cell reorientation behaviors under substrate stretching, in which the mechano-chemical coupling at molecular, subcellular, and cellular scales was considered. The effect of matrix stiffness was also considered in a FEM based mechano-chemical coupling simulation. We showed that the collapsing time of focal adhesion decreases with the increasing of the loading frequency, however, the cell reorientation time exhibits a biphasic frequency-dependent behavior. Our results suggested that this biphasic behavior might be caused by the competition between the frequency-dependent collapsing of focal adhesions and the less frequency-dependent formation of stress fibers aligning away from the loading direction. At the low loading frequency, the collapsing of focal adhesion dominates the reorientation process, however, at the high loading frequency the polymerization of stress fiber dominates the reorientation. Moreover, we showed that the compliance of matrix may help accelerate the cell reorientation because focal adhesion is prone to be instable on soft matrix. |
| 关键词 | Cell Adhesion
Mechanosensitivity
Stress Fiber
Mechano-chemical Coupling
Multiscale Modeling
Loading Frequency
Smooth-muscle-cells
Stress Fibers
Orientation Response
Mechanical Force
Kinematic Model
Catch Bonds
Dynamics
Actin
Integrin
Mechanosensitivity
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| 学科领域 | Cell Biology
; Biophysics
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| DOI | 10.1007/s12195-011-0187-6
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| URL | 查看原文
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| 收录类别 | SCI
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| 语种 | 英语
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| WOS记录号 | WOS:000297866300012
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| 关键词[WOS] | SMOOTH-MUSCLE-CELLS
; STRESS FIBERS
; ORIENTATION RESPONSE
; MECHANICAL FORCE
; KINEMATIC MODEL
; CATCH BONDS
; DYNAMICS
; ACTIN
; INTEGRIN
; MECHANOSENSITIVITY
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| WOS研究方向 | Cell Biology
; Biophysics
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| WOS类目 | Cell & Tissue Engineering
; Biophysics
; Cell Biology
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| 项目资助者 | This research was supported by the National Natural Science Foundation of China through Grant No. 10732050, 10872115 and 11025208. LD acknowledges the support from the key project of Chinese Academy of Sciences through KJCX2-YW-M04 and KJCX-SW-L08.
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| 课题组名称 | LNM冲击动力学与新型材料力学性能
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| 论文分区 | Q4
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/44908
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| 专题 | 非线性力学国家重点实验室
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| 通讯作者 | Ji, BH (reprint author), Beijing Inst Technol, Biomech & Biomat Lab, Dept Appl Mech, Beijing 100081, Peoples R China |
推荐引用方式
GB/T 7714 |
Zhong Y,Kong D,Dai LH,et al. Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching[J]. Cellular and Molecular Bioengineering,2011,4,3,:442-456.
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| APA |
Zhong Y,孔冬,戴兰宏,季葆华,&Ji, BH .(2011).Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching.Cellular and Molecular Bioengineering,4(3),442-456.
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| MLA |
Zhong Y,et al."Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching".Cellular and Molecular Bioengineering 4.3(2011):442-456.
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