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Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching
Zhong Y; Kong D(孔冬); Dai LH(戴兰宏); Ji BH(季葆华); Ji, BH (reprint author), Beijing Inst Technol, Biomech & Biomat Lab, Dept Appl Mech, Beijing 100081, Peoples R China
Source PublicationCellular and Molecular Bioengineering
2011
Volume4Issue:3Pages:442-456
ISSN1865-5025
AbstractThe adhesion-based cell mechanosensitivity plays central roles in many physiological and pathological processes. Recently, quantitative understanding of cell responses to external force has been intensively pursued. However, the frequency dependent cell responses to the substrate stretching have not yet been fully understood. Here we developed a multiscale modeling framework for studying cell reorientation behaviors under substrate stretching, in which the mechano-chemical coupling at molecular, subcellular, and cellular scales was considered. The effect of matrix stiffness was also considered in a FEM based mechano-chemical coupling simulation. We showed that the collapsing time of focal adhesion decreases with the increasing of the loading frequency, however, the cell reorientation time exhibits a biphasic frequency-dependent behavior. Our results suggested that this biphasic behavior might be caused by the competition between the frequency-dependent collapsing of focal adhesions and the less frequency-dependent formation of stress fibers aligning away from the loading direction. At the low loading frequency, the collapsing of focal adhesion dominates the reorientation process, however, at the high loading frequency the polymerization of stress fiber dominates the reorientation. Moreover, we showed that the compliance of matrix may help accelerate the cell reorientation because focal adhesion is prone to be instable on soft matrix.
KeywordCell Adhesion Mechanosensitivity Stress Fiber Mechano-chemical Coupling Multiscale Modeling Loading Frequency Smooth-muscle-cells Stress Fibers Orientation Response Mechanical Force Kinematic Model Catch Bonds Dynamics Actin Integrin Mechanosensitivity
Subject AreaCell Biology ; Biophysics
DOI10.1007/s12195-011-0187-6
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000297866300012
WOS KeywordSMOOTH-MUSCLE-CELLS ; STRESS FIBERS ; ORIENTATION RESPONSE ; MECHANICAL FORCE ; KINEMATIC MODEL ; CATCH BONDS ; DYNAMICS ; ACTIN ; INTEGRIN ; MECHANOSENSITIVITY
WOS Research AreaCell Biology ; Biophysics
WOS SubjectCell & Tissue Engineering ; Biophysics ; Cell Biology
Funding OrganizationThis research was supported by the National Natural Science Foundation of China through Grant No. 10732050, 10872115 and 11025208. LD acknowledges the support from the key project of Chinese Academy of Sciences through KJCX2-YW-M04 and KJCX-SW-L08.
DepartmentLNM冲击动力学与新型材料力学性能
ClassificationQ4
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/44908
Collection非线性力学国家重点实验室
Corresponding AuthorJi, BH (reprint author), Beijing Inst Technol, Biomech & Biomat Lab, Dept Appl Mech, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Zhong Y,Kong D,Dai LH,et al. Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching[J]. Cellular and Molecular Bioengineering,2011,4(3):442-456.
APA Zhong Y,孔冬,戴兰宏,季葆华,&Ji, BH .(2011).Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching.Cellular and Molecular Bioengineering,4(3),442-456.
MLA Zhong Y,et al."Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching".Cellular and Molecular Bioengineering 4.3(2011):442-456.
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