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Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry
Zhang YB; Chen YY(陈艳艳); Jin G(靳刚); Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China
Source PublicationSensors and Actuators B-Chemical
2011
Volume159Issue:1Pages:121-125
ISSN0925-4005
AbstractWe describe highly sensitive, label-free, real time detection of the biomarker CA19-9 using microarray biosensors based on total internal reflection imaging ellipsometry (TIRIE), in which anti-CA19-9 is immobilized onto the PEGylated phospholipid membrane. Strong resistance against non-specific adsorption of PEGylated lipid membrane allows direct serum assay without the blocking agent. We find that PEG density has an influence on antibody assembly and only a proper amount of PEG is appropriate for antigen binding. Two different methods for antibody assembly are compared and finally protein A is preferred due to its higher protein binding efficiency. The estimated detection limit of CA19-9 is 18.2 U/ml, which is lower than the cut-off value for normal level (37 U/ml). Regression analysis between the results of microarray biosensor and electrochemiluminescence immunoassay (ECLIA) suggests a high correlation (R = 0.989). The capability for real-time monitoring of tumor marker with high sensitivity and selectivity in clinically relevant samples opens up substantial possibilities for early cancer diagnosis and treatment. (C) 2011 Elsevier B.V. All rights reserved.
KeywordPegylated Phospholipid Membrane Total Internal Reflection Imaging Ellipsometry Ca19-9 Resectable Pancreatic Adenocarcinoma Perioperative Ca19-9 Levels (18)Fdg-pet Examination Predict Stage Protein Adsorption Immobilization Surfaces Antigen Cancer
Subject AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
DOI10.1016/j.snb.2011.06.059
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000295745900017
WOS KeywordRESECTABLE PANCREATIC ADENOCARCINOMA ; PERIOPERATIVE CA19-9 LEVELS ; (18)FDG-PET EXAMINATION ; PREDICT STAGE ; PROTEIN ; ADSORPTION ; IMMOBILIZATION ; SURFACES ; ANTIGEN ; CANCER
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
Funding OrganizationThe authors would like to gratefully acknowledge financial supports from Ministry of Science and Technology of China (2008AA02Z419, 2009CB320302), Natural Science Foundation (10804083, 20845003) of China, Chinese Academy of Sciences (KJCX2-YW-M04, KJCX2-YW-M03) and Key Laboratory of Microgravity, Institute of Mechanics, CAS.
DepartmentNML纳米生物光学
Classification二类/Q1
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45066
Collection国家微重力实验室
Corresponding AuthorChen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Zhang YB,Chen YY,Jin G,et al. Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry[J]. Sensors and Actuators B-Chemical,2011,159(1):121-125.
APA Zhang YB,陈艳艳,靳刚,&Chen, YY .(2011).Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry.Sensors and Actuators B-Chemical,159(1),121-125.
MLA Zhang YB,et al."Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry".Sensors and Actuators B-Chemical 159.1(2011):121-125.
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