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Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay
Fu ZL(傅长亮); Tong CF(佟春芳); Wang ML; Gao YX(高宇欣); Zhang Y(章燕); Lv SQ(吕守芹); Liang SL; Dong C; Long M(龙勉); Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
2011
发表期刊Journal of Biological Chemistry
卷号286期号:40页码:34777-34787
ISSN0021-9258
摘要Interactions between polymorphonuclear neutrophils (PMNs) and tumor cells have been reported to facilitate the adhesion and subsequent extravasation of tumor cells through the endothelium under blood flow, both of which are mediated by binding beta(2)-integrin to intercellular adhesion molecule 1 (ICAM-1). Here the adhesions between humanWM9 metastatic melanoma cells, PMNs, and human pulmonary microvascular endothelial cells (HPMECs) were quantified by a gas-driven micropipette aspiration technique (GDMAT). Our data indicated that the cellular binding affinity of PMN-WM9 pair was 3.9-fold higher than that of the PMN-HPMEC pair. However, the effective binding affinities per molecular pair were comparable between the two cell pairs no matter whether WM9 cells or HPMECs were quiescent or cytokine-activated, indicating that the stronger adhesion between PMN-WM9 pair is mainly attributed to the high expression of ICAM-1 on WM9 cells. These results proposed an alternative mechanism, where WM9 melanoma cells adhere first with PMNs near vessel-wall regions and then bind to endothelial cells via PMNs under blood flow. In contrast, the adhesions between human MDA-MB-231 metastatic breast carcinoma cells and PMNs showed a comparable cellular binding affinity to PMN-HPMEC pair because the ICAM-1 expressions on MDA-MB-231 cells and HPMECs are similar. Furthermore, differences were observed in the intrinsic forward and reverse rates of the beta(2)-integrin-ICAM-1 bond between PMN-TC and PMN-EC pairs. This GDMAT assay enables us to quantify the binding kinetics of cell adhesion molecules physiologically expressed on nucleated cells. The findings also further the understanding of leukocyte-facilitated tumor cell adhesion from the viewpoint of molecular binding kinetics.
关键词Mammary Adenocarcinoma Cells Hydrodynamic Shear Melanoma Adhesion 2-dimensional Kinetics Human Neutrophils P-selectin Metastasis Development Ligand Interactions Concurrent Binding Cancer Metastasis
学科领域Biochemistry & Molecular Biology
DOI10.1074/jbc.M111.281642
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000295406300032
关键词[WOS]MAMMARY ADENOCARCINOMA CELLS ; HYDRODYNAMIC SHEAR ; MELANOMA ADHESION ; 2-DIMENSIONAL KINETICS ; HUMAN NEUTROPHILS ; P-SELECTIN ; METASTASIS DEVELOPMENT ; LIGAND INTERACTIONS ; CONCURRENT BINDING ; CANCER METASTASIS
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
项目资助者This work was supported, in whole or in part, by National Institutes of Health Grants CA-97306, CA-125707, M01-RR-010732, and C06-RR-016499. This work was also supported by National Natural Science Foundation of China Grants 30730032, 10702075, and 10902117; National Key Basic Research Foundation of China Grants 2006CB910303 and 2011CB710904; Strategic Priority Research Program of Chinese Academy of Science grants XDA01030102 and XDA04020219, Knowledge Innovation Program of Chinese Academy of Science Grant KJCX2-YW-L08 (to M.L.), and National Science Foundation Grants BES-0138474 and CBET-0729091 (to C.D.).
课题组名称NML分子-细胞生物力学与空间生命科学
论文分区二类/Q2
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/45069
专题国家微重力实验室
通讯作者Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Fu ZL,Tong CF,Wang ML,et al. Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay[J]. Journal of Biological Chemistry,2011,286(40):34777-34787.
APA 傅长亮.,佟春芳.,Wang ML.,高宇欣.,章燕.,...&Long, MA .(2011).Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay.Journal of Biological Chemistry,286(40),34777-34787.
MLA 傅长亮,et al."Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay".Journal of Biological Chemistry 286.40(2011):34777-34787.
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