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Title:
Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay
Author: Fu ZL(傅长亮); Tong CF(佟春芳); Wang ML; Gao YX(高宇欣); Zhang Y(章燕); Lv SQ(吕守芹); Liang SL; Dong C; Long M(龙勉)
Source: Journal of Biological Chemistry
Issued Date: 2011
Volume: 286, Issue:40, Pages:34777-34787
Abstract: Interactions between polymorphonuclear neutrophils (PMNs) and tumor cells have been reported to facilitate the adhesion and subsequent extravasation of tumor cells through the endothelium under blood flow, both of which are mediated by binding beta(2)-integrin to intercellular adhesion molecule 1 (ICAM-1). Here the adhesions between humanWM9 metastatic melanoma cells, PMNs, and human pulmonary microvascular endothelial cells (HPMECs) were quantified by a gas-driven micropipette aspiration technique (GDMAT). Our data indicated that the cellular binding affinity of PMN-WM9 pair was 3.9-fold higher than that of the PMN-HPMEC pair. However, the effective binding affinities per molecular pair were comparable between the two cell pairs no matter whether WM9 cells or HPMECs were quiescent or cytokine-activated, indicating that the stronger adhesion between PMN-WM9 pair is mainly attributed to the high expression of ICAM-1 on WM9 cells. These results proposed an alternative mechanism, where WM9 melanoma cells adhere first with PMNs near vessel-wall regions and then bind to endothelial cells via PMNs under blood flow. In contrast, the adhesions between human MDA-MB-231 metastatic breast carcinoma cells and PMNs showed a comparable cellular binding affinity to PMN-HPMEC pair because the ICAM-1 expressions on MDA-MB-231 cells and HPMECs are similar. Furthermore, differences were observed in the intrinsic forward and reverse rates of the beta(2)-integrin-ICAM-1 bond between PMN-TC and PMN-EC pairs. This GDMAT assay enables us to quantify the binding kinetics of cell adhesion molecules physiologically expressed on nucleated cells. The findings also further the understanding of leukocyte-facilitated tumor cell adhesion from the viewpoint of molecular binding kinetics.
Keyword: Mammary Adenocarcinoma Cells ; Hydrodynamic Shear ; Melanoma Adhesion ; 2-Dimensional Kinetics ; Human Neutrophils ; P-Selectin ; Metastasis Development ; Ligand Interactions ; Concurrent Binding ; Cancer Metastasis
Language: 英语
Indexed Type: SCI
Corresponding Author: Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
Correspondent Email: mlong@imech.ac.cn
DOI: 10.1074/jbc.M111.281642
Related URLs: 查看原文
DOC Type: Article
WOS Subject: Biochemistry & Molecular Biology
WOS Subject Extended: Biochemistry & Molecular Biology
WOS Keyword Plus: MAMMARY ADENOCARCINOMA CELLS ; HYDRODYNAMIC SHEAR ; MELANOMA ADHESION ; 2-DIMENSIONAL KINETICS ; HUMAN NEUTROPHILS ; P-SELECTIN ; METASTASIS DEVELOPMENT ; LIGAND INTERACTIONS ; CONCURRENT BINDING ; CANCER METASTASIS
WOS ID: WOS:000295406300032
ISSN: 0021-9258
Rank: [Fu, Changliang; Tong, Chunfang; Wang, Manliu; Gao, Yuxin; Zhang, Yan; Lu, Shouqin; Long, Mian] Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China; [Fu, Changliang; Tong, Chunfang; Wang, Manliu; Gao, Yuxin; Zhang, Yan; Lu, Shouqin; Long, Mian] Chinese Acad Sci, Natl Micrograv Lab, Beijing 100190, Peoples R China; [Fu, Changliang; Tong, Chunfang; Wang, Manliu; Gao, Yuxin; Zhang, Yan; Lu, Shouqin; Long, Mian] Chinese Acad Sci, Ctr Biomech & Bioengn, Inst Mech, Beijing 100190, Peoples R China; [Liang, Shile; Dong, Cheng] Penn State Univ, Dept Bioengn, University Pk, PA 16802 USA
Subject: Biochemistry & Molecular Biology
Department: NML分子-细胞生物力学与空间生命科学
Classification: 二类/Q2
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/45069
Appears in Collections:国家微重力实验室_期刊论文

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Recommended Citation:
Fu ZL,Tong CF,Wang ML,et al. Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay[J]. Journal of Biological Chemistry,2011-01-01,286(40):34777-34787.
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