| Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay |
| Fu ZL(傅长亮) ; Tong CF(佟春芳); Wang ML; Gao YX(高宇欣); Zhang Y(章燕); Lv SQ(吕守芹); Liang SL; Dong C; Long M(龙勉); Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
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| 发表期刊 | Journal of Biological Chemistry
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| 2011
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| 卷号 | 286期号:40页码:34777-34787 |
| ISSN | 0021-9258
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| 摘要 | Interactions between polymorphonuclear neutrophils (PMNs) and tumor cells have been reported to facilitate the adhesion and subsequent extravasation of tumor cells through the endothelium under blood flow, both of which are mediated by binding beta(2)-integrin to intercellular adhesion molecule 1 (ICAM-1). Here the adhesions between humanWM9 metastatic melanoma cells, PMNs, and human pulmonary microvascular endothelial cells (HPMECs) were quantified by a gas-driven micropipette aspiration technique (GDMAT). Our data indicated that the cellular binding affinity of PMN-WM9 pair was 3.9-fold higher than that of the PMN-HPMEC pair. However, the effective binding affinities per molecular pair were comparable between the two cell pairs no matter whether WM9 cells or HPMECs were quiescent or cytokine-activated, indicating that the stronger adhesion between PMN-WM9 pair is mainly attributed to the high expression of ICAM-1 on WM9 cells. These results proposed an alternative mechanism, where WM9 melanoma cells adhere first with PMNs near vessel-wall regions and then bind to endothelial cells via PMNs under blood flow. In contrast, the adhesions between human MDA-MB-231 metastatic breast carcinoma cells and PMNs showed a comparable cellular binding affinity to PMN-HPMEC pair because the ICAM-1 expressions on MDA-MB-231 cells and HPMECs are similar. Furthermore, differences were observed in the intrinsic forward and reverse rates of the beta(2)-integrin-ICAM-1 bond between PMN-TC and PMN-EC pairs. This GDMAT assay enables us to quantify the binding kinetics of cell adhesion molecules physiologically expressed on nucleated cells. The findings also further the understanding of leukocyte-facilitated tumor cell adhesion from the viewpoint of molecular binding kinetics. |
| 关键词 | Mammary Adenocarcinoma Cells
Hydrodynamic Shear
Melanoma Adhesion
2-dimensional Kinetics
Human Neutrophils
P-selectin
Metastasis Development
Ligand Interactions
Concurrent Binding
Cancer Metastasis
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| 学科领域 | Biochemistry & Molecular Biology
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| DOI | 10.1074/jbc.M111.281642
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| URL | 查看原文
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| 收录类别 | SCI
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| 语种 | 英语
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| WOS记录号 | WOS:000295406300032
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| 关键词[WOS] | MAMMARY ADENOCARCINOMA CELLS
; HYDRODYNAMIC SHEAR
; MELANOMA ADHESION
; 2-DIMENSIONAL KINETICS
; HUMAN NEUTROPHILS
; P-SELECTIN
; METASTASIS DEVELOPMENT
; LIGAND INTERACTIONS
; CONCURRENT BINDING
; CANCER METASTASIS
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| WOS研究方向 | Biochemistry & Molecular Biology
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| WOS类目 | Biochemistry & Molecular Biology
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| 项目资助者 | This work was supported, in whole or in part, by National Institutes of Health Grants CA-97306, CA-125707, M01-RR-010732, and C06-RR-016499. This work was also supported by National Natural Science Foundation of China Grants 30730032, 10702075, and 10902117; National Key Basic Research Foundation of China Grants 2006CB910303 and 2011CB710904; Strategic Priority Research Program of Chinese Academy of Science grants XDA01030102 and XDA04020219, Knowledge Innovation Program of Chinese Academy of Science Grant KJCX2-YW-L08 (to M.L.), and National Science Foundation Grants BES-0138474 and CBET-0729091 (to C.D.).
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| 课题组名称 | NML分子-细胞生物力学与空间生命科学
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| 论文分区 | 二类/Q2
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/45069
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| 专题 | 微重力重点实验室
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| 通讯作者 | Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China |
推荐引用方式
GB/T 7714 |
Fu ZL,Tong CF,Wang ML,et al. Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay[J]. Journal of Biological Chemistry,2011,286,40,:34777-34787.
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| APA |
傅长亮.,佟春芳.,Wang ML.,高宇欣.,章燕.,...&Long, MA .(2011).Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay.Journal of Biological Chemistry,286(40),34777-34787.
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| MLA |
傅长亮,et al."Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay".Journal of Biological Chemistry 286.40(2011):34777-34787.
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