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Title: Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow
Author: Fu ZL(傅长亮); Tong CF(佟春芳); Dong C; Long M(龙勉)
Source: Cellular and Molecular Bioengineering
Issued Date: 2011
Volume: 4, Issue:3, Pages:427-441
Abstract: Shear-induced cell aggregation and disaggregation, governed by specific receptor-ligand binding, play important roles in many biological and biophysical processes. While a lot of studies have focused on elucidating the shear rate and shear stress dependence of cell aggregation, the majority of existing models based on population balance equation (PBE) has rarely dealt with cell aggregation dynamics upon intrinsic molecular kinetics. Here, a kinetic model was developed for further understanding cell aggregation and disaggregation in a linear shear flow. The novelty of the model is that a set of simple equations was constructed by coupling two-body collision theory with receptor-ligand binding kinetics. Two cases of study were employed to validate the model: one is for the homotypic aggregation dynamics of latex beads cross-linked by protein G-IgG binding, and the other is for the heterotypic aggregation dynamics of neutrophils-tumor cells governed by beta 2-integrin-ligand interactions. It was found that the model fits the data well and the obtained kinetic parameters are consistent with the previous predictions and experimental measurements. Moreover, the decay factor defined biophysically to account for the chemokine- and shear-induced regulation of receptor and/or ligand expression and conformation was compared at molecular and cellular levels. Our results provided a universal framework to quantify the molecular kinetics of receptor-ligand binding in shear-induced cell aggregation dynamics.
Keyword: Two-Dimensional Kinetics ; Cone-Plate Viscometer ; Homotypic Aggregation ; Heterotypic Aggregation ; Bell Model ; Protein G-Igg Bond ; Beta(2)-Integrin And Icam-1 Bond ; Human Blood-Platelets ; Intercellular-Adhesion Molecule-1 ; Hydrodynamic Shear ; Disaggregation Kinetics ; Neutrophil Aggregation ; Sequential Binding ; Mediated Adhesion ; Stable Adhesion ; Melanoma-Cells ; Latex Spheres
Language: 英语
Indexed Type: SCI
Corresponding Author: Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China
Correspondent Email: mlong@imech.ac.cn
DOI: 10.1007/s12195-011-0167-x
Related URLs: 查看原文
DOC Type: Article
WOS Subject: Cell & Tissue Engineering ; Biophysics ; Cell Biology
WOS Subject Extended: Cell Biology ; Biophysics
WOS Keyword Plus: HUMAN BLOOD-PLATELETS ; INTERCELLULAR-ADHESION MOLECULE-1 ; HYDRODYNAMIC SHEAR ; DISAGGREGATION KINETICS ; NEUTROPHIL AGGREGATION ; SEQUENTIAL BINDING ; MEDIATED ADHESION ; STABLE ADHESION ; MELANOMA-CELLS ; LATEX SPHERES
WOS ID: WOS:000297866300011
ISSN: 1865-5025
Rank: [Fu, Changliang; Tong, Chunfang; Long, Mian] Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China; [Fu, Changliang; Tong, Chunfang; Long, Mian] Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, Beijing 100190, Peoples R China; [Fu, Changliang; Tong, Chunfang; Long, Mian] Chinese Acad Sci, Ctr Biomech & Bioengn, Inst Mech, Beijing 100190, Peoples R China; [Dong, Cheng] Penn State Univ, Dept Bioengn, University Pk, PA 16802 USA
Subject: Cell Biology; Biophysics
Department: NML分子-细胞生物力学与空间生命科学
Classification: Q4
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/45075
Appears in Collections:国家微重力实验室_期刊论文

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Recommended Citation:
傅长亮;佟春芳;Dong C;龙勉.Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow,CELLULAR AND MOLECULAR BIOENGINEERING,2011,4(3):427-441
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