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Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow
Fu ZL(傅长亮); Tong CF(佟春芳); Dong C; Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China
2011
发表期刊Cellular and Molecular Bioengineering
卷号4期号:3页码:427-441
ISSN1865-5025
摘要Shear-induced cell aggregation and disaggregation, governed by specific receptor-ligand binding, play important roles in many biological and biophysical processes. While a lot of studies have focused on elucidating the shear rate and shear stress dependence of cell aggregation, the majority of existing models based on population balance equation (PBE) has rarely dealt with cell aggregation dynamics upon intrinsic molecular kinetics. Here, a kinetic model was developed for further understanding cell aggregation and disaggregation in a linear shear flow. The novelty of the model is that a set of simple equations was constructed by coupling two-body collision theory with receptor-ligand binding kinetics. Two cases of study were employed to validate the model: one is for the homotypic aggregation dynamics of latex beads cross-linked by protein G-IgG binding, and the other is for the heterotypic aggregation dynamics of neutrophils-tumor cells governed by beta 2-integrin-ligand interactions. It was found that the model fits the data well and the obtained kinetic parameters are consistent with the previous predictions and experimental measurements. Moreover, the decay factor defined biophysically to account for the chemokine- and shear-induced regulation of receptor and/or ligand expression and conformation was compared at molecular and cellular levels. Our results provided a universal framework to quantify the molecular kinetics of receptor-ligand binding in shear-induced cell aggregation dynamics.
关键词Two-dimensional Kinetics Cone-plate Viscometer Homotypic Aggregation Heterotypic Aggregation Bell Model Protein G-igg Bond Beta(2)-integrin And Icam-1 Bond Human Blood-platelets Intercellular-adhesion Molecule-1 Hydrodynamic Shear Disaggregation Kinetics Neutrophil Aggregation Sequential Binding Mediated Adhesion Stable Adhesion Melanoma-cells Latex Spheres
学科领域Cell Biology ; Biophysics
DOI10.1007/s12195-011-0167-x
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收录类别SCI
语种英语
WOS记录号WOS:000297866300011
关键词[WOS]HUMAN BLOOD-PLATELETS ; INTERCELLULAR-ADHESION MOLECULE-1 ; HYDRODYNAMIC SHEAR ; DISAGGREGATION KINETICS ; NEUTROPHIL AGGREGATION ; SEQUENTIAL BINDING ; MEDIATED ADHESION ; STABLE ADHESION ; MELANOMA-CELLS ; LATEX SPHERES
WOS研究方向Cell Biology ; Biophysics
WOS类目Cell & Tissue Engineering ; Biophysics ; Cell Biology
项目资助者This work was supported by National Natural Science Foundation of China grants 30730032, 11072251, 10902117, and 10702075, Chinese Academy of Sciences grants KJCX2-YW-L08 and Y2010030, and National Key Basic Research Foundation of China grant 2011CB710904.
课题组名称NML分子-细胞生物力学与空间生命科学
论文分区Q4
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/45075
专题国家微重力实验室
通讯作者Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China
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GB/T 7714
Fu ZL,Tong CF,Dong C,et al. Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow[J]. Cellular and Molecular Bioengineering,2011,4(3):427-441.
APA 傅长亮,佟春芳,Dong C,龙勉,&Long, M .(2011).Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow.Cellular and Molecular Bioengineering,4(3),427-441.
MLA 傅长亮,et al."Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow".Cellular and Molecular Bioengineering 4.3(2011):427-441.
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