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Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor
Zhang YB; Chen YY(陈艳艳); Jin G(靳刚); Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China
Source PublicationApplied Surface Science
2011
Volume257Issue:22Pages:9407-9413
ISSN0169-4332
AbstractChitosan cushion can support the air stability of phospholipid membrane, but the problem of serum solubility of phospholipid membrane prevents it from use in serum detection applications. Poly (ethylene glycol) (PEG) shielding promises both stability and non-specific adsorption resistance for phospholipid membrane. An air stable phospholipid membrane microarray has been successfully fabricated on chitosan modified silicon wafer. We have demonstrated the potential application of PEGylated phospholipid membrane in imaging ellipsometry-based protein biosensor. Because of the strong resistance against non-specific adsorption of serum, antigens are immobilized onto the membrane surface through chemical activation and further bind their antibodies without using blocking agent. Taking advantage of the multiple and parallel reaction capabilities of microfluidic reactor system, we have assayed the binding by varying both the density of antigen on the membrane surface and the concentration of antibody in solution. (C) 2011 Elsevier B. V. All rights reserved.
KeywordPhospholipid Membrane Chitosan Imaging Ellipsometry Biosensor Afm Self-assembled Monolayers Supported Lipid-bilayers Nonspecific Protein Adsorption Surface-plasmon Resonance Molecular-conformation Cell-membrane Resist Cholesterol Adhesion Microarrays
Subject AreaChemistry ; Materials Science ; Physics
DOI10.1016/j.apsusc.2011.06.019
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000293590300031
WOS KeywordSELF-ASSEMBLED MONOLAYERS ; SUPPORTED LIPID-BILAYERS ; NONSPECIFIC PROTEIN ADSORPTION ; SURFACE-PLASMON RESONANCE ; MOLECULAR-CONFORMATION ; CELL-MEMBRANE ; RESIST ; CHOLESTEROL ; ADHESION ; MICROARRAYS
WOS Research AreaChemistry ; Materials Science ; Physics
WOS SubjectChemistry, Physical ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
Funding OrganizationThe authors would like to gratefully acknowledge financial supports from Ministry of Science and Technology of China (2008AA02Z419, 2009CB320302), Natural Science Foundation (10804083, 20845003) of China, and Key Laboratory of Microgravity, Institute of Mechanics, CAS.
DepartmentNML纳米生物光学
Classification一类
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45076
Collection国家微重力实验室
Corresponding AuthorChen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Zhang YB,Chen YY,Jin G,et al. Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor[J]. Applied Surface Science,2011,257(22):9407-9413.
APA Zhang YB,陈艳艳,靳刚,&Chen, YY .(2011).Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor.Applied Surface Science,257(22),9407-9413.
MLA Zhang YB,et al."Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor".Applied Surface Science 257.22(2011):9407-9413.
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