|Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor|
|Zhang YB; Chen YY(陈艳艳); Jin G(靳刚); Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China
|Source Publication||Applied Surface Science
|Abstract||Chitosan cushion can support the air stability of phospholipid membrane, but the problem of serum solubility of phospholipid membrane prevents it from use in serum detection applications. Poly (ethylene glycol) (PEG) shielding promises both stability and non-specific adsorption resistance for phospholipid membrane. An air stable phospholipid membrane microarray has been successfully fabricated on chitosan modified silicon wafer. We have demonstrated the potential application of PEGylated phospholipid membrane in imaging ellipsometry-based protein biosensor. Because of the strong resistance against non-specific adsorption of serum, antigens are immobilized onto the membrane surface through chemical activation and further bind their antibodies without using blocking agent. Taking advantage of the multiple and parallel reaction capabilities of microfluidic reactor system, we have assayed the binding by varying both the density of antigen on the membrane surface and the concentration of antibody in solution. (C) 2011 Elsevier B. V. All rights reserved.|
Nonspecific Protein Adsorption
; Materials Science
|WOS Keyword||SELF-ASSEMBLED MONOLAYERS
; SUPPORTED LIPID-BILAYERS
; NONSPECIFIC PROTEIN ADSORPTION
; SURFACE-PLASMON RESONANCE
|WOS Research Area||Chemistry
; Materials Science
|WOS Subject||Chemistry, Physical
; Materials Science, Coatings & Films
; Physics, Applied
; Physics, Condensed Matter
|Funding Organization||The authors would like to gratefully acknowledge financial supports from Ministry of Science and Technology of China (2008AA02Z419, 2009CB320302), Natural Science Foundation (10804083, 20845003) of China, and Key Laboratory of Microgravity, Institute of Mechanics, CAS.
|Corresponding Author||Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China|
Zhang YB,Chen YY,Jin G,et al. Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor[J]. Applied Surface Science,2011,257(22):9407-9413.
Zhang YB,陈艳艳,靳刚,&Chen, YY .(2011).Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor.Applied Surface Science,257(22),9407-9413.
Zhang YB,et al."Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor".Applied Surface Science 257.22(2011):9407-9413.