| Probe stiffness regulates receptor-ligand bond lifetime under force |
| Zhang Y(章燕) ; Lv SQ(吕守芹); Long M(龙勉); Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
|
| 发表期刊 | Science China-Physics Mechanics & Astronomy
 |
| 2011
|
| 卷号 | 54期号:5页码:923-929 |
| ISSN | 1674-7348
|
| 摘要 | Receptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently attracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quantified the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5x10(-3) to 4.7x10(-2) pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k >= 3.0x10(-2) pN/nm. This work furthered the understanding of the forced dissociation of selectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukocytes under blood flow. |
| 关键词 | Probe Stiffness
Bond Lifetime
Optical Trap
Selectin Glycoprotein Ligand-1
P-selectin
Cell-adhesion
Binding-kinetics
Leukocytes
Molecule
Psgl-1
Flow
|
| 学科领域 | Physics
|
| DOI | 10.1007/s11433-011-4302-4
|
| URL | 查看原文
|
| 收录类别 | SCI
; EI
|
| 语种 | 英语
|
| WOS记录号 | WOS:000290768300022
|
| 关键词[WOS] | SELECTIN GLYCOPROTEIN LIGAND-1
; P-SELECTIN
; CELL-ADHESION
; BINDING-KINETICS
; LEUKOCYTES
; MOLECULE
; PSGL-1
; FLOW
|
| WOS研究方向 | Physics
|
| WOS类目 | Physics, Multidisciplinary
|
| 课题组名称 | NML分子-细胞生物力学与空间生命科学
|
| 论文分区 | 二类/Q3
|
| 引用统计 |
|
| 文献类型 | 期刊论文
|
| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/45085
|
| 专题 | 微重力重点实验室
|
| 通讯作者 | Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China |
推荐引用方式
GB/T 7714 |
Zhang Y,Lv SQ,Long M,et al. Probe stiffness regulates receptor-ligand bond lifetime under force[J]. Science China-Physics Mechanics & Astronomy,2011,54,5,:923-929.
|
| APA |
章燕,吕守芹,龙勉,&Long, MA .(2011).Probe stiffness regulates receptor-ligand bond lifetime under force.Science China-Physics Mechanics & Astronomy,54(5),923-929.
|
| MLA |
章燕,et al."Probe stiffness regulates receptor-ligand bond lifetime under force".Science China-Physics Mechanics & Astronomy 54.5(2011):923-929.
|
修改评论