Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)

doi:10.1016/j.tsf.2010.12.130

IMECH-IR > 微重力重点实验室

	Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)
	Liu L(刘丽); Chen YY(陈艳艳); Meng YH(孟永宏); Chen S(陈涉); Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
发表期刊	Thin Solid Films
	2011
卷号	519 期号:9 页码:2758-2762
ISSN	0040-6090
摘要	The biosensor based on the total internal reflection imaging ellipsometry (TIRIE) is realized as an automatic analysis method for protein interaction processes in real-time, with high throughput and label-free. An evanescent wave is used as the optical probe to monitor bio-molecular interactions on a chip surface with a high sensitivity due to its phase sensitive property. In this paper, the technique is optimized with a polarization setting, a spectroscopic light source and a low noise CCD detector to improve the performance of the biosensor in sensitivity and detection limit, as evidenced by a quantitative detection of Hepatitis B virus surface antigen (HbsAg) with concentrations of 8, 16, 32, 64, 125 and 250 ng/ml. The sensitivity is increased by one order of magnitude and the detection limit has been extended more than 50 times for HbsAg detection. (C) 2011 Elsevier B.V. All rights reserved.
关键词	Sensitivity Improvement Imaging Ellipsometry Total Internal Reflection Biosensor Hbsag Detection Plasmon Enhanced Ellipsometry Surface Resonance Sensors
学科领域	Materials Science ; Physics
DOI	10.1016/j.tsf.2010.12.130
URL	查看原文
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000289174200041
关键词[WOS]	PLASMON ENHANCED ELLIPSOMETRY ; SURFACE ; RESONANCE ; SENSORS
WOS研究方向	Materials Science ; Physics
WOS类目	Materials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
课题组名称	NML纳米生物光学
论文分区	二类/Q2
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/45100
专题	微重力重点实验室
通讯作者	Jin, G (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu L,Chen YY,Meng YH,et al. Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)[J]. Thin Solid Films,2011,519,9,:2758-2762.
APA	Liu L,Chen YY,Meng YH,Chen S,Jin G,&Jin, G .(2011).Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE).Thin Solid Films,519(9),2758-2762.
MLA	Liu L,et al."Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)".Thin Solid Films 519.9(2011):2758-2762.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
SCI-J2011168.pdf（612KB）			开放获取	--	浏览下载