|Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry|
|Niu Y(牛宇); Zhuang J; Liu L(刘丽); Yan XY; Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
|Source Publication||Thin Solid Films
|Abstract||In order to meet the requirements of antibody comparison and evaluation, the optimization for the surface modification, ligand immobilization, unspecific blocking and ligand receptor interaction condition is introduced to the biosensor based on imaging ellipsometry (BIE), so that it may serve as an antibody screening platform to evaluate antibody antigen interactions. Two kinds of anti-ricin antibody named pVHHS1 and 5S1R are immobilized in a patterned array format on the silicon surface modified with succinic anhydride, respectively, to form a sensing surface. Ricin and its similar toxin abrin as the reference in solution are delivered, respectively, on the patterned sensing surface for anti-ricin antibody and ricin interaction. The limit of detection value of ricin detected by pVHHS1 and 5S1R is obtained as 1 mu g/ml and 5 ng/ml, respectively. Results show that pVHHS1 and 5S1R specifically bind with ricin but not with abrin, which is in accordance with ELISA's result. (C) 2010 Elsevier B.V. All rights reserved.|
|Subject Area||Materials Science
|WOS Keyword||NEUTRALIZING MONOCLONAL-ANTIBODY
; OPTICAL PROTEIN-CHIP
; SILICON SURFACE
|WOS Research Area||Materials Science
|WOS Subject||Materials Science, Multidisciplinary
; Materials Science, Coatings & Films
; Physics, Applied
; Physics, Condensed Matter
|Corresponding Author||Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China|
Niu Y,Zhuang J,Liu L,et al. Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry[J]. Thin Solid Films,2011,519(9):2768-2771.
牛宇,Zhuang J,刘丽,Yan XY,靳刚,&Jin, G .(2011).Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry.Thin Solid Films,519(9),2768-2771.
牛宇,et al."Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry".Thin Solid Films 519.9(2011):2768-2771.