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Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry
Niu Y(牛宇); Zhuang J; Liu L(刘丽); Yan XY; Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
Source PublicationThin Solid Films
2011
Volume519Issue:9Pages:2768-2771
ISSN0040-6090
AbstractIn order to meet the requirements of antibody comparison and evaluation, the optimization for the surface modification, ligand immobilization, unspecific blocking and ligand receptor interaction condition is introduced to the biosensor based on imaging ellipsometry (BIE), so that it may serve as an antibody screening platform to evaluate antibody antigen interactions. Two kinds of anti-ricin antibody named pVHHS1 and 5S1R are immobilized in a patterned array format on the silicon surface modified with succinic anhydride, respectively, to form a sensing surface. Ricin and its similar toxin abrin as the reference in solution are delivered, respectively, on the patterned sensing surface for anti-ricin antibody and ricin interaction. The limit of detection value of ricin detected by pVHHS1 and 5S1R is obtained as 1 mu g/ml and 5 ng/ml, respectively. Results show that pVHHS1 and 5S1R specifically bind with ricin but not with abrin, which is in accordance with ELISA's result. (C) 2010 Elsevier B.V. All rights reserved.
KeywordRicin Detection Antigen-antibody Interaction Biosensor Imaging Ellipsometry Neutralizing Monoclonal-antibody Optical Protein-chip Silicon Surface Immobilization Visualization System Cells Assay
Subject AreaMaterials Science ; Physics
DOI10.1016/j.tsf.2010.12.054
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000289174200043
WOS KeywordNEUTRALIZING MONOCLONAL-ANTIBODY ; OPTICAL PROTEIN-CHIP ; SILICON SURFACE ; IMMOBILIZATION ; VISUALIZATION ; SYSTEM ; CELLS ; ASSAY
WOS Research AreaMaterials Science ; Physics
WOS SubjectMaterials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
DepartmentNML纳米生物光学
Classification二类/Q2
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45102
Collection国家微重力实验室
Corresponding AuthorJin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Niu Y,Zhuang J,Liu L,et al. Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry[J]. Thin Solid Films,2011,519(9):2768-2771.
APA 牛宇,Zhuang J,刘丽,Yan XY,靳刚,&Jin, G .(2011).Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry.Thin Solid Films,519(9),2768-2771.
MLA 牛宇,et al."Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry".Thin Solid Films 519.9(2011):2768-2771.
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