A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection

doi:10.1039/c0lc00341g

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	A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection
	Liu JS; Wang JY; Chen ZG; Yu Y(于泳); Yang XJ; Zhang XB; Xu Z; Liu, JS (reprint author), Dalian Univ Technol, Key Lab Micro Nano Technol & Syst Liaoning Prov, Dalian 116023, Liaoning, Peoples R China
发表期刊	Lab on A Chip
	2011
卷号	11 期号:5 页码:969-973
ISSN	1473-0197
摘要	A three-layer poly (methyl methacrylate) (PMMA) electrophoresis microchip integrated with Pt microelectrodes for contactless conductivity detection is presented. A 50 mu m-thick PMMA film is used as the insulating layer and placed between the channel plate (containing the microchannel) and the electrode plate (containing the microelectrode). The three-layer structure facilitates the achievement of a thin insulating layer, obviates the difficulty of integrating microelectrodes on a thin film, and does not compromise the integration of microchips. To overcome the thermal and chemical incompatibilities of polymers and photolithographic techniques, a modified lift-off process was developed to integrate Pt microelectrodes onto the PMMA substrate. A novel two-step bonding method was created to assemble the complete PMMA microchip. A low limit of detection of 1.25 mu g ml(-1) for Na(+) and high separation efficiency of 77 000 and 48 000 plates/m for Na(+) and K(+) were obtained when operating the detector at a low excitation frequency of 60 kHz.
关键词	Capillary-electrophoresis Fabrication System Polymerization Electrodes Devices Chip
学科领域	Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
DOI	10.1039/c0lc00341g
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000287409600028
关键词[WOS]	CAPILLARY-ELECTROPHORESIS ; FABRICATION ; SYSTEM ; POLYMERIZATION ; ELECTRODES ; DEVICES ; CHIP
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS类目	Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
项目资助者	This work was supported by the National Natural Science Foundation of China (50605006, 51075056, 50705012, 20890024, 20727006), and the National Basic Research Program of China (2007CB714502). We thank Yanan Du, Jason W. Nichol and Seila Selimovic for scientific discussions and revisions. J. Liu, Y. Yu, Z. Chen, Z. Xu and C. Liu designed the research strategy; J. Liu, J. Wang, X. Yang and X. Zhang performed the experiments; Y. Yu designed the microchip; J. Liu, J. Wang and X. Yang wrote the paper.
课题组名称	NML复杂流体
论文分区	一类
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/45108
专题	微重力重点实验室
通讯作者	Liu, JS (reprint author), Dalian Univ Technol, Key Lab Micro Nano Technol & Syst Liaoning Prov, Dalian 116023, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Liu JS,Wang JY,Chen ZG,et al. A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection[J]. Lab on A Chip,2011,11,5,:969-973.
APA	Liu JS.,Wang JY.,Chen ZG.,Yu Y.,Yang XJ.,...&Liu, JS .(2011).A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection.Lab on A Chip,11(5),969-973.
MLA	Liu JS,et al."A three-layer PMMA electrophoresis microchip with Pt microelectrodes insulated by a thin film for contactless conductivity detection".Lab on A Chip 11.5(2011):969-973.

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