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Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States
Dai GL(戴国亮); Sazaki Gen; Matsui Takuro; Tsukamoto Katsuo; Nakajima Kazuo; Kang Q(康琦); Hu WR(胡文瑞); Sazaki, G (reprint author), Hokkaido Univ, Inst Low Temp Sci, N19-W8 Kita Ku, Sapporo, Hokkaido 0600819, Japan
Source PublicationCrystal Growth & Design
2011
Volume11Issue:1Pages:88-92
ISSN1528-7483
AbstractThe adsorption/desorption behavior of mobile solute molecules at a solution-crystal interface has been explored using crystals of model protein hen egg-white lysozyme (HEWL) and fluorescent-labeled HEWL (F-HEWL) molecules. We have tracked the transient processes occurring during adsorption/desorption of identical F-HEWL molecules on a tetragonal HEWL crystal surface by single-molecule visualization using a total internal reflection fluorescent microscope and pulsed discontinuous laser illumination. We found an induction period (similar to 70 min) after which the number density of F-HEWL molecules adsorbed mainly on steps increased linearly with the adsorption time. We show direct evidence that the residence time of molecules on the crystal surface gradually increases during the transition process from a solute species to the crystal after successive multistep processes.
KeywordReflection Fluorescence Microscopy Single-protein Molecules Crystals Adsorption Diffusion Interface Surface
Subject AreaChemistry ; Crystallography ; Materials Science
DOI10.1021/cg100869r
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000285735100015
WOS KeywordREFLECTION FLUORESCENCE MICROSCOPY ; SINGLE-PROTEIN MOLECULES ; CRYSTALS ; ADSORPTION ; DIFFUSION ; INTERFACE ; SURFACE
WOS Research AreaChemistry ; Crystallography ; Materials Science
WOS SubjectChemistry, Multidisciplinary ; Crystallography ; Materials Science, Multidisciplinary
Funding OrganizationThe authors thank H. Higuchi (The University of Tokyo) for valuable discussions, and they acknowledge grants from the National Basic Research Program of China (973 Program) (G.D.: No. 2011CB710901), the Knowledge Innovation Program of the Chinese Academy of Sciences (G.D.: No. KJCX2-YW-L08), and the National Nature Science Foundation of China (G.D.: No. 20603043), and Grants-in-Aid (G.S.: Nos. 17034007 and 18360003) from the Scientific Research of the Ministry of Education, Science and Culture, Japan. One of the authors (G.D.) acknowledges a grant from Tohoku University as a visiting professor. This work was carried out as a part of "Project Research B" at the Center for Interdisciplinary Research, Tohoku University, as well as the "Ground-based Research Announcement for Space Utilization" of the Japan Space Forum.
DepartmentNML复杂流体
Classification一类
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45110
Collection国家微重力实验室
Corresponding AuthorSazaki, G (reprint author), Hokkaido Univ, Inst Low Temp Sci, N19-W8 Kita Ku, Sapporo, Hokkaido 0600819, Japan
Recommended Citation
GB/T 7714
Dai GL,Sazaki Gen,Matsui Takuro,et al. Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States[J]. Crystal Growth & Design,2011,11(1):88-92.
APA 戴国亮.,Sazaki Gen.,Matsui Takuro.,Tsukamoto Katsuo.,Nakajima Kazuo.,...&Sazaki, G .(2011).Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States.Crystal Growth & Design,11(1),88-92.
MLA 戴国亮,et al."Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States".Crystal Growth & Design 11.1(2011):88-92.
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