Approach to Quantitative Detection of CD146 with the Label-free Protein Biosensor Based on Imaging Ellipsometry

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	Approach to Quantitative Detection of CD146 with the Label-free Protein Biosensor Based on Imaging Ellipsometry
	Niu Y(牛宇); Liu L(刘丽); Yan XY; Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
会议录名称	SENSORS, CAMERAS, AND SYSTEMS FOR INDUSTRIAL, SCIENTIFIC, AND CONSUMER APPLICATIONS XII
	2011
页码	78750R
会议名称	Conference on Sensors, Cameras, and Systems for Industrial, Scientific, and Consumer Applications XII
会议日期	JAN 25-27, 2011
会议地点	San Francisco, CA
摘要	CD146 glycoprotein belonging to cell adhesion molecules is considered to be a novel target on endothelial cell involved in tumor angiogenesis. The biosensor based on imaging ellipsometry (BIE) which is performed in null and off-null mode is used for CD146 detection as a trial by the following steps. Firstly, anti-CD146 antibody as ligand is immobilized on Protein G modified silicon substrate. Then, CD146 test is carried out and its calibration curve is established for the requirement of quantitative detection. Finally, 18 serum samples are detected quantitatively and their results are validated by ELISA's. The sensitivity for CD146 detection achieves the order of ng/ml and the relationship between BIE signal y (grayscale value) and CD146 concentration x (ng/ml) is y=3.3ln(x) +91.3. Compared with ELISA's, the majority of results are in agreement, and the results of two approaches have significant statistic relevance.
关键词	Cd146 Detection Biosensor Imaging Ellipsometry Mel-cam Antibody Visualization Growth Cancer
WOS记录号	WOS:000299998500249
课题组名称	NML纳米生物光学
ISBN号	978-0-8194-8412-3
URL	查看原文
收录类别	CPCI-S
语种	英语
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	会议论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/45315
专题	微重力重点实验室
通讯作者	Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Niu Y,Liu L,Yan XY,et al. Approach to Quantitative Detection of CD146 with the Label-free Protein Biosensor Based on Imaging Ellipsometry[C]SENSORS, CAMERAS, AND SYSTEMS FOR INDUSTRIAL, SCIENTIFIC, AND CONSUMER APPLICATIONS XII,2011:78750R.

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