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Approach to Quantitative Detection of CD146 with the Label-free Protein Biosensor Based on Imaging Ellipsometry
Niu Y(牛宇); Liu L(刘丽); Yan XY; Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
Source PublicationSENSORS, CAMERAS, AND SYSTEMS FOR INDUSTRIAL, SCIENTIFIC, AND CONSUMER APPLICATIONS XII
2011
Pages78750R
Conference NameConference on Sensors, Cameras, and Systems for Industrial, Scientific, and Consumer Applications XII
Conference DateJAN 25-27, 2011
Conference PlaceSan Francisco, CA
AbstractCD146 glycoprotein belonging to cell adhesion molecules is considered to be a novel target on endothelial cell involved in tumor angiogenesis. The biosensor based on imaging ellipsometry (BIE) which is performed in null and off-null mode is used for CD146 detection as a trial by the following steps. Firstly, anti-CD146 antibody as ligand is immobilized on Protein G modified silicon substrate. Then, CD146 test is carried out and its calibration curve is established for the requirement of quantitative detection. Finally, 18 serum samples are detected quantitatively and their results are validated by ELISA's. The sensitivity for CD146 detection achieves the order of ng/ml and the relationship between BIE signal y (grayscale value) and CD146 concentration x (ng/ml) is y=3.3ln(x) +91.3. Compared with ELISA's, the majority of results are in agreement, and the results of two approaches have significant statistic relevance.
KeywordCd146 Detection Biosensor Imaging Ellipsometry Mel-cam Antibody Visualization Growth Cancer
WOS IDWOS:000299998500249
DepartmentNML纳米生物光学
ISBN978-0-8194-8412-3
URL查看原文
Indexed ByCPCI-S
Language英语
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type会议论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45315
Collection国家微重力实验室
Corresponding AuthorJin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Niu Y,Liu L,Yan XY,et al. Approach to Quantitative Detection of CD146 with the Label-free Protein Biosensor Based on Imaging Ellipsometry[C]SENSORS, CAMERAS, AND SYSTEMS FOR INDUSTRIAL, SCIENTIFIC, AND CONSUMER APPLICATIONS XII,2011:78750R.
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