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Probe stiffness regulates receptor-ligand bond lifetime under force
Zhang Y(章燕); Lv SQ(吕守芹); Long M(龙勉); Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
2011
会议名称Symposium on Multiscale Modeling and Simulation of Materials/11th International Conference in Asia of the International-Union-of-Materials-Research-Societies
会议日期SEP 25-28, 2010
会议地点Qingdao, PEOPLES R CHINA
会议录名称SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
页码923-929
摘要Receptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently attracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quantified the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5x10(-3) to 4.7x10(-2) pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k >= 3.0x10(-2) pN/nm. This work furthered the understanding of the forced dissociation of selectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukocytes under blood flow.
关键词Probe Stiffness Bond Lifetime Optical Trap
课题组名称中科院国家微重力实验室
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收录类别CPCI(ISTP)
语种英语
文献类型会议论文
条目标识符http://dspace.imech.ac.cn/handle/311007/45325
专题国家微重力实验室
通讯作者Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Zhang Y,Lv SQ,Long M,et al. Probe stiffness regulates receptor-ligand bond lifetime under force[C]. 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA:SCIENCE CHINA PRESS,2011:923-929.
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