IMECH-IR  > 国家微重力实验室
Probe stiffness regulates receptor-ligand bond lifetime under force
Zhang Y(章燕); Lv SQ(吕守芹); Long M(龙勉); Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
Conference NameSymposium on Multiscale Modeling and Simulation of Materials/11th International Conference in Asia of the International-Union-of-Materials-Research-Societies
Conference DateSEP 25-28, 2010
Conference PlaceQingdao, PEOPLES R CHINA
AbstractReceptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently attracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quantified the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5x10(-3) to 4.7x10(-2) pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k >= 3.0x10(-2) pN/nm. This work furthered the understanding of the forced dissociation of selectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukocytes under blood flow.
KeywordProbe Stiffness Bond Lifetime Optical Trap
Indexed ByCPCI(ISTP)
Document Type会议论文
Corresponding AuthorLong, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang Y,Lv SQ,Long M,et al. Probe stiffness regulates receptor-ligand bond lifetime under force[C]SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2011:923-929.
Files in This Item: Download All
File Name/Size DocType Version Access License
Conf2011-096.pdf(534KB) 开放获取--View Download
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[章燕]'s Articles
[吕守芹]'s Articles
[龙勉]'s Articles
Baidu academic
Similar articles in Baidu academic
[章燕]'s Articles
[吕守芹]'s Articles
[龙勉]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[章燕]'s Articles
[吕守芹]'s Articles
[龙勉]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Conf2011-096.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.