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Title: Probe stiffness regulates receptor-ligand bond lifetime under force
Author: Zhang Y(章燕) ; Lv SQ(吕守芹) ; Long M(龙勉)
Issued Date: 2011
Conference Name: Symposium on Multiscale Modeling and Simulation of Materials/11th International Conference in Asia of the International-Union-of-Materials-Research-Societies
Conference Date: SEP 25-28, 2010
Conference Place: Qingdao, PEOPLES R CHINA
Abstract: Receptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently attracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quantified the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5x10(-3) to 4.7x10(-2) pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k >= 3.0x10(-2) pN/nm. This work furthered the understanding of the forced dissociation of selectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukocytes under blood flow.
Keyword: probe stiffness ; bond lifetime ; optical trap
Indexed Type: CPCI(ISTP)
Subject: Physics, Multidisciplinary
Corresponding Author: Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
Related URLs: 查看原文
Source: SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
Publisher: SCIENCE CHINA PRESS
Publish Place: 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA
Language: 英语
Department: 中科院国家微重力实验室
Content Type: 会议论文
URI: http://dspace.imech.ac.cn/handle/311007/45325
Appears in Collections:国家微重力实验室_会议论文

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SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2011,54(5):923-929.
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