IMECH-IR  > 国家微重力实验室
Probe stiffness regulates receptor-ligand bond lifetime under force
Zhang Y(章燕); Lv SQ(吕守芹); Long M(龙勉); Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
Source PublicationSCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
2011
Pages923-929
Conference NameSymposium on Multiscale Modeling and Simulation of Materials/11th International Conference in Asia of the International-Union-of-Materials-Research-Societies
Conference DateSEP 25-28, 2010
Conference PlaceQingdao, PEOPLES R CHINA
AbstractReceptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently attracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quantified the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5x10(-3) to 4.7x10(-2) pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k >= 3.0x10(-2) pN/nm. This work furthered the understanding of the forced dissociation of selectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukocytes under blood flow.
KeywordProbe Stiffness Bond Lifetime Optical Trap
Department中科院国家微重力实验室
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Indexed ByCPCI(ISTP)
Language英语
Document Type会议论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45325
Collection国家微重力实验室
Corresponding AuthorLong, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang Y,Lv SQ,Long M,et al. Probe stiffness regulates receptor-ligand bond lifetime under force[C]. 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA:SCIENCE CHINA PRESS,2011:923-929.
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