IMECH-IR  > 国家微重力实验室
Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry
Niu Y(牛宇); Zhuang J; Liu L(刘丽); Yan XY; Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
Source PublicationTHIN SOLID FILMS
2011
Pages2768-2771
Conference Name5th International Conference on Spectroscopic Ellipsometry
Conference DateMAY 23-29, 2010
Conference PlaceAlbany, NY
AbstractIn order to meet the requirements of antibody comparison and evaluation, the optimization for the surface modification, ligand immobilization, unspecific blocking and ligand receptor interaction condition is introduced to the biosensor based on imaging ellipsometry (BIE), so that it may serve as an antibody screening platform to evaluate antibody antigen interactions. Two kinds of anti-ricin antibody named pVHHS1 and 5S1R are immobilized in a patterned array format on the silicon surface modified with succinic anhydride, respectively, to form a sensing surface. Ricin and its similar toxin abrin as the reference in solution are delivered, respectively, on the patterned sensing surface for anti-ricin antibody and ricin interaction. The limit of detection value of ricin detected by pVHHS1 and 5S1R is obtained as 1 mu g/ml and 5 ng/ml, respectively. Results show that pVHHS1 and 5S1R specifically bind with ricin but not with abrin, which is in accordance with ELISA's result. (C) 2010 Elsevier B.V. All rights reserved.
KeywordRicin Detection Antigen-antibody Interaction Biosensor Imaging Ellipsometry
Department中科院国家微重力实验室
Funding OrganizationUniv Albany, Coll Nanoscale & Engn
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Indexed ByCPCI(ISTP)
Language英语
Document Type会议论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/45328
Collection国家微重力实验室
Corresponding AuthorJin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Niu Y,Zhuang J,Liu L,et al. Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry[C]. PO BOX 564, 1001 LAUSANNE, SWITZERLAND:ELSEVIER SCIENCE SA,2011:2768-2771.
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