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Investigation of the interaction between a bivalent aptamer and thrombin by AFM
Ge L; Jin G(靳刚); Fang XH; Jin, G; Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China.
Source PublicationLANGMUIR
2012
Volume28Issue:1Pages:707-713
ISSN0743-7463
AbstractAptamers are a new class of molecular probes for protein recognition, detection, and inhibition. Multivalent aptamer protein binding through aptamer assembly has been currently developed as an effective way to achieve higher protein affinity and selectivity. In this study, the specific interaction between bivalent aptamer Bi-8S and thrombin has been measured directly and quantitatively by atomic force microscopy to investigate the unbinding dynamics and dissociation energy landscape of the multivalent interaction. Bivalent aptamer Bi-8S contains thrombin's two aptamers, 15apt and 27apt, which are linked by eight spacer phosphoramidites. The results revealed the sequential dissociation of the two aptamers. Moreover, the dynamic force spectroscopy data revealed that the 27apt's binding to the thrombin remains largely unaffected by the eight-spacer phosphoramidites within Bi-8S. In contrast, the eight-spacer phosphoramidites stabilized the 15apt-thrombin binding.
KeywordDynamic Force Spectroscopy Single-molecule Energy Landscape Sinorhizobium-meliloti Membrane-proteins Receptor-binding Dna-molecules Rna Ligands Inhibitor Recognition
Subject Area生物力学
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Indexed BySCI ; EI
Language英语
WOS IDWOS:000298904900089
Funding OrganizationWe thank Le Mu for experimental advice and Mehdi Damaghi and Prof. Daniel Muller for helpful discussions. This work was supported by the Chinese Academy of Sciences and the National Basic Research Program of China (2011CB911001) and the NSFC (no. 20821003).
DepartmentNML纳米生物光学
Classification一类
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/46555
Collection国家微重力实验室
Corresponding AuthorJin, G; Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China.
Recommended Citation
GB/T 7714
Ge L,Jin G,Fang XH,et al. Investigation of the interaction between a bivalent aptamer and thrombin by AFM[J]. LANGMUIR,2012,28(1):707-713.
APA Ge L,靳刚,Fang XH,Jin, G,&Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China..(2012).Investigation of the interaction between a bivalent aptamer and thrombin by AFM.LANGMUIR,28(1),707-713.
MLA Ge L,et al."Investigation of the interaction between a bivalent aptamer and thrombin by AFM".LANGMUIR 28.1(2012):707-713.
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