Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow

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	Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow
	Lu XL; Huo B(霍波); Chiang V; Guo XE; Guo, XE; Columbia Univ, Dept Biomed Engn, Bone Bioenn Lab, 351 Engn Terrace,Mail Code 8904,1210 Amsterdam Av, New York, NY 10027 USA.
发表期刊	JOURNAL OF BONE AND MINERAL RESEARCH
	2012-03-01
卷号	27 期号:3 页码:563-574
ISSN	0884-0431
摘要	Osteocytes, regarded as the mechanical sensor in bone, respond to mechanical stimulation by activating biochemical pathways and mediating the cellular activities of other bone cells. Little is known about how osteocytic networks respond to physiological mechanical stimuli. In this study, we compared the mechanical sensitivity of osteocytic and osteoblastic networks under physiological-related fluid shear stress (0.5 to 4?Pa). The intracellular calcium ([Ca2+]i) responses in micropatterned in vitro osteoblastic or osteocytic networks were recorded and analyzed. Osteocytes in the network showed highly repetitive spikelike [Ca2+]i peaks under fluid flow stimulation, which are dramatically different from those in the osteoblastic network. The number of responsive osteocytes in the network remained at a constant high percentage (>95%) regardless of the magnitude of shear stress, whereas the number of responsive osteoblasts in the network significantly depends on the strength of fluid flow. All spatiotemporal parameters of calcium signaling demonstrated that osteocytic networks are more sensitive and dynamic than osteoblastic networks, especially under low-level mechanical stimulations. Furthermore, pathway studies were performed to identify the molecular mechanisms responsible for the differences in [Ca2+]i signaling between osteoblastic and osteocytic networks. The results suggested that the T-type voltage-gated calcium channels (VGCC) expressed on osteocytes may play an essential role in the unique kinetics of [Ca2+]i signaling in osteocytic networks, whereas the L-type VGCC is critical for both types of cells to release multiple [Ca2+]i peaks. The extracellular calcium source and intracellular calcium store in ER-, ATP-, PGE2-, NO-, and caffeine-related pathways are found to play similar roles in the [Ca2+]i signaling for both osteoblasts and osteocytes. The findings in this study proved that osteocytic networks possess unique characteristics in sensing and processing mechanical signals.
关键词	Osteocytes Osteoblasts Cell Network Mechanotransduction Fluid Flow Calcium Signaling Functional Gap-junctions Bone Cell Networks Mc3t3-e1 Osteoblasts Nitric-oxide Shear-stress Prostaglandin Release Mlo-y4 Osteocytes Ca2++ Channels Atp Release Long Bones
学科领域	生物力学
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000300682500007
项目资助者	This work was supported by National Institutes of Health (NIH) grants R21 AR052417, R01 AR052461, and RC1 AR058453 (XEG). We acknowledge the contribution of Dr. PuiLeng Isabel Leong for her help in PLC inhibition experiments. We thank Dr. L Bonewald for her generous gift of MLO-Y4 cells.
课题组名称	NML分子-细胞生物力学与空间生命科学
论文分区	二类/Q1
引用统计	被引频次：96[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/46560
专题	微重力重点实验室
通讯作者	Guo, XE; Columbia Univ, Dept Biomed Engn, Bone Bioenn Lab, 351 Engn Terrace,Mail Code 8904,1210 Amsterdam Av, New York, NY 10027 USA.
推荐引用方式 GB/T 7714	Lu XL,Huo B,Chiang V,et al. Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow[J]. JOURNAL OF BONE AND MINERAL RESEARCH,2012,27,3,:563-574.
APA	Lu XL,Huo B,Chiang V,Guo XE,Guo, XE,&Columbia Univ, Dept Biomed Engn, Bone Bioenn Lab, 351 Engn Terrace,Mail Code 8904,1210 Amsterdam Av, New York, NY 10027 USA..(2012).Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow.JOURNAL OF BONE AND MINERAL RESEARCH,27(3),563-574.
MLA	Lu XL,et al."Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow".JOURNAL OF BONE AND MINERAL RESEARCH 27.3(2012):563-574.

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