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Title:
Spreading of human neutrophils on an ICAM-1-immobilized substrate under shear flow
Author: Zhan DY(展冬颖); Zhang Y(章燕); Long M(龙勉)
Source: CHINESE SCIENCE BULLETIN
Issued Date: 2012-03-01
Volume: 57, Issue:7, Pages:769-775
Abstract: Neutrophil (PMN) spreading on endothelium, mediated by the interactions between surface-bound beta 2 integrin and intercellular adhesion molecule-1 (ICAM-1) in the inflammatory cascade, is crucial for PMN post-adhesion and trans-migration in blood flow. The underlying mechanisms by which shear flow regulates PMN spreading dynamics are not well understood. Here, a parallel-plate flow chamber assay was applied to quantify the time course of PMN adhesion and spreading on an ICAM-1-immobilized substrate. Two types of shear flow, steady flows at shear stresses of 0.2, 0.5, and 1 dyne/cm(2) and stepwise flows at 0, 1, and 10 dyne/cm(2), were used to elucidate the impact of shear flow on cell adhesion and spreading. The number of adhered PMNs, the fraction of spreading PMNs and the projected area of spread PMNs were determined and were found to correlate with the distribution of surface-bound beta 2 integrin subunit (CD11a, CD11b, or CD18). The results indicate that PMN spreading on an ICAM-1 substrate is bi-directionally regulated under shear flow. CD11a, CD11b and CD18 subunits of beta 2 integrin contribute distinctly to PMN spreading on ICAM-1 substrates. This work provides new insights into understanding PMN spreading on the endothelium, mediated by beta 2 integrin and ICAM-1 under shear flow.
Keyword: neutrophil ; spreading ; beta 2 integrin ; ICAM-1 ; shear stress ; Cell-Surface Adhesion ; L-Selectin ; Polymorphonuclear Leukocytes ; Endothelial-Cells ; Rolling Adhesions ; P-Selectin ; Integrin ; Affinity ; Stress ; ICAM-1
Language: 英语
Indexed Type: SCI
Corresponding Author: Long, MA ; Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China.
Correspondent Email: mlong@imech.ac.cn
Related URLs: 查看原文
WOS ID: WOS:000300771200011
ISSN: 1001-6538
Rank: [Zhan Dongyan; Zhang Yan; Long Mian] Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China;Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China
Subject: 交叉与边缘领域的力学::生物力学
Department: NML分子-细胞生物力学与空间生命科学
Classification: 二类
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/46561
Appears in Collections:国家微重力实验室_期刊论文

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Recommended Citation:
Zhan DY,Zhang Y,Long M. Spreading of human neutrophils on an ICAM-1-immobilized substrate under shear flow[J]. CHINESE SCIENCE BULLETIN,2012-03-01,57(7):769-775.
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