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Spreading of human neutrophils on an ICAM-1-immobilized substrate under shear flow
Zhan DY(展冬颖); Zhang Y(章燕); Long M(龙勉); Long, MA; Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China.
2012-03-01
发表期刊CHINESE SCIENCE BULLETIN
卷号57期号:7页码:769-775
ISSN1001-6538
摘要Neutrophil (PMN) spreading on endothelium, mediated by the interactions between surface-bound beta 2 integrin and intercellular adhesion molecule-1 (ICAM-1) in the inflammatory cascade, is crucial for PMN post-adhesion and trans-migration in blood flow. The underlying mechanisms by which shear flow regulates PMN spreading dynamics are not well understood. Here, a parallel-plate flow chamber assay was applied to quantify the time course of PMN adhesion and spreading on an ICAM-1-immobilized substrate. Two types of shear flow, steady flows at shear stresses of 0.2, 0.5, and 1 dyne/cm(2) and stepwise flows at 0, 1, and 10 dyne/cm(2), were used to elucidate the impact of shear flow on cell adhesion and spreading. The number of adhered PMNs, the fraction of spreading PMNs and the projected area of spread PMNs were determined and were found to correlate with the distribution of surface-bound beta 2 integrin subunit (CD11a, CD11b, or CD18). The results indicate that PMN spreading on an ICAM-1 substrate is bi-directionally regulated under shear flow. CD11a, CD11b and CD18 subunits of beta 2 integrin contribute distinctly to PMN spreading on ICAM-1 substrates. This work provides new insights into understanding PMN spreading on the endothelium, mediated by beta 2 integrin and ICAM-1 under shear flow.
关键词Neutrophil Spreading Beta 2 Integrin Icam-1 Shear Stress Cell-surface Adhesion L-selectin Polymorphonuclear Leukocytes Endothelial-cells Rolling Adhesions P-selectin Integrin Affinity Stress Icam-1
学科领域生物力学
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收录类别SCI
语种英语
WOS记录号WOS:000300771200011
项目资助者The authors are grateful to Dr. Quhuan Li of the South China University of Technology for technical assistance and helpful discussions. This work was supported by the National Natural Science Foundation of China (30730032 and 10902117), Chinese Academy of Sciences Knowledge Innovation Project (KJCX2-YW-L08 and Y2010030), and the National Basic Research Program of China (2011CB710904).
课题组名称NML分子-细胞生物力学与空间生命科学
论文分区二类
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/46561
专题国家微重力实验室
通讯作者Long, MA; Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China.
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Zhan DY,Zhang Y,Long M,et al. Spreading of human neutrophils on an ICAM-1-immobilized substrate under shear flow[J]. CHINESE SCIENCE BULLETIN,2012,57(7):769-775.
APA 展冬颖,章燕,龙勉,Long, MA,&Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China..(2012).Spreading of human neutrophils on an ICAM-1-immobilized substrate under shear flow.CHINESE SCIENCE BULLETIN,57(7),769-775.
MLA 展冬颖,et al."Spreading of human neutrophils on an ICAM-1-immobilized substrate under shear flow".CHINESE SCIENCE BULLETIN 57.7(2012):769-775.
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