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Fluid flow-induced calcium response in early or late differentiated osteoclasts
Li P(李平); Hu M(胡漫); Sun SJ(孙树津); Zhang Y(章燕); Gao YX(高宇欣); Long M(龙勉); Huo B(霍波); Zhang D; Huo, B; Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, 15 N 4th Ring Rd, Beijing 100190, Peoples R China.
2012-09-01
发表期刊ANNALS OF BIOMEDICAL ENGINEERING
卷号40期号:9页码:1874-1883
ISSN0090-6964
摘要Intracellular calcium oscillation caused by receptor activator of nuclear factor kappa-B ligand has been demonstrated to promote the differentiation of osteoclasts. Osteoclasts are recruited on the surface of trabeculae, and are exposed to fluid flow caused by the deformation of the bone matrix. However, the roles of fluid shear stress (FSS) on calcium response during the differentiation process of osteoclasts are still unknown. In the current study, the formation of tartrate-resistant acid phosphatase-positive, multinucleated osteoclasts from RAW264.7 macrophage cells were induced by co-culturing them with the conditioned medium from MC3T3-E1 osteoblasts. The in situ observations showed a high correlation between the area and the nuclear number of osteoclasts. The cells were stimulated by FSS at different levels (1 or 10 dyne/cm(2)) before (0 day) or after being induced for 4 or 8 days. The mechanically-induced calcium response was recorded and analyzed. The results indicated a different property of calcium oscillation for the osteoclasts in different fusion stages (i.e., more calcium-responsive peaks appeared in small osteoclasts than those in the larger ones). The rates of calcium influx decreased and the time of recovery in osteoclast cytosol increased along with the fusion of osteoclasts. In addition, increasing the FSS level enhanced the calcium oscillation of osteoclasts at early induction (4 days). However, this effect was weakened at the late induction (8 days). The present work could help provide understanding regarding the mechanism of the involvement of calcium in mechanically induced bone remodeling.
关键词Osteoclasts Fluid Shear Stress Calcium Oscillation Induction Time Differentiation Mechanical Strain Rat Osteoclasts Bone-resorption Activation Expression Cells Ca2++
学科领域生物力学
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收录类别SCI ; EI
语种英语
WOS记录号WOS:000307400900003
项目资助者This work was supported by the National Natural Science Foundation of China [30970707 (BH) and 31070829 (DZ)], the National Key Basic Research Foundation of China (2011CB710904), and the Knowledge Innovation Project of the CAS (KJCX2-YW-L08) (ML).
课题组名称NML分子-细胞生物力学与空间生命科学
论文分区二类/Q2
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/46584
专题国家微重力实验室
通讯作者Huo, B; Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, 15 N 4th Ring Rd, Beijing 100190, Peoples R China.
推荐引用方式
GB/T 7714
Li P,Hu M,Sun SJ,et al. Fluid flow-induced calcium response in early or late differentiated osteoclasts[J]. ANNALS OF BIOMEDICAL ENGINEERING,2012,40(9):1874-1883.
APA Li P.,Hu M.,Sun SJ.,Zhang Y.,Gao YX.,...&Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, 15 N 4th Ring Rd, Beijing 100190, Peoples R China..(2012).Fluid flow-induced calcium response in early or late differentiated osteoclasts.ANNALS OF BIOMEDICAL ENGINEERING,40(9),1874-1883.
MLA Li P,et al."Fluid flow-induced calcium response in early or late differentiated osteoclasts".ANNALS OF BIOMEDICAL ENGINEERING 40.9(2012):1874-1883.
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