IMECH-IR  > 国家微重力实验室
Fluid flow-induced calcium response in early or late differentiated osteoclasts
Li P(李平); Hu M(胡漫); Sun SJ(孙树津); Zhang Y(章燕); Gao YX(高宇欣); Long M(龙勉); Huo B(霍波); Zhang D; Huo, B; Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, 15 N 4th Ring Rd, Beijing 100190, Peoples R China.
Source PublicationANNALS OF BIOMEDICAL ENGINEERING
2012-09-01
Volume40Issue:9Pages:1874-1883
ISSN0090-6964
AbstractIntracellular calcium oscillation caused by receptor activator of nuclear factor kappa-B ligand has been demonstrated to promote the differentiation of osteoclasts. Osteoclasts are recruited on the surface of trabeculae, and are exposed to fluid flow caused by the deformation of the bone matrix. However, the roles of fluid shear stress (FSS) on calcium response during the differentiation process of osteoclasts are still unknown. In the current study, the formation of tartrate-resistant acid phosphatase-positive, multinucleated osteoclasts from RAW264.7 macrophage cells were induced by co-culturing them with the conditioned medium from MC3T3-E1 osteoblasts. The in situ observations showed a high correlation between the area and the nuclear number of osteoclasts. The cells were stimulated by FSS at different levels (1 or 10 dyne/cm(2)) before (0 day) or after being induced for 4 or 8 days. The mechanically-induced calcium response was recorded and analyzed. The results indicated a different property of calcium oscillation for the osteoclasts in different fusion stages (i.e., more calcium-responsive peaks appeared in small osteoclasts than those in the larger ones). The rates of calcium influx decreased and the time of recovery in osteoclast cytosol increased along with the fusion of osteoclasts. In addition, increasing the FSS level enhanced the calcium oscillation of osteoclasts at early induction (4 days). However, this effect was weakened at the late induction (8 days). The present work could help provide understanding regarding the mechanism of the involvement of calcium in mechanically induced bone remodeling.
KeywordOsteoclasts Fluid Shear Stress Calcium Oscillation Induction Time Differentiation Mechanical Strain Rat Osteoclasts Bone-resorption Activation Expression Cells Ca2++
Subject Area生物力学
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000307400900003
Funding OrganizationThis work was supported by the National Natural Science Foundation of China [30970707 (BH) and 31070829 (DZ)], the National Key Basic Research Foundation of China (2011CB710904), and the Knowledge Innovation Project of the CAS (KJCX2-YW-L08) (ML).
DepartmentNML分子-细胞生物力学与空间生命科学
Classification二类/Q2
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/46584
Collection国家微重力实验室
Corresponding AuthorHuo, B; Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, 15 N 4th Ring Rd, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Li P,Hu M,Sun SJ,et al. Fluid flow-induced calcium response in early or late differentiated osteoclasts[J]. ANNALS OF BIOMEDICAL ENGINEERING,2012,40(9):1874-1883.
APA Li P.,Hu M.,Sun SJ.,Zhang Y.,Gao YX.,...&Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, 15 N 4th Ring Rd, Beijing 100190, Peoples R China..(2012).Fluid flow-induced calcium response in early or late differentiated osteoclasts.ANNALS OF BIOMEDICAL ENGINEERING,40(9),1874-1883.
MLA Li P,et al."Fluid flow-induced calcium response in early or late differentiated osteoclasts".ANNALS OF BIOMEDICAL ENGINEERING 40.9(2012):1874-1883.
Files in This Item: Download All
File Name/Size DocType Version Access License
SCI-J2012-082.pdf(887KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li P(李平)]'s Articles
[Hu M(胡漫)]'s Articles
[Sun SJ(孙树津)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li P(李平)]'s Articles
[Hu M(胡漫)]'s Articles
[Sun SJ(孙树津)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li P(李平)]'s Articles
[Hu M(胡漫)]'s Articles
[Sun SJ(孙树津)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: SCI-J2012-082.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.