中国科学院力学研究所机构知识库
Advanced  
IMECH-IR  > 国家微重力实验室  > 期刊论文
Title:
Fluid flow-induced calcium response in early or late differentiated osteoclasts
Author: Li P(李平); Hu M(胡漫); Sun SJ(孙树津); Zhang Y(章燕); Gao YX(高宇欣); Long M(龙勉); Huo B(霍波); Zhang D
Source: ANNALS OF BIOMEDICAL ENGINEERING
Issued Date: 2012-09-01
Volume: 40, Issue:9, Pages:1874-1883
Abstract: Intracellular calcium oscillation caused by receptor activator of nuclear factor kappa-B ligand has been demonstrated to promote the differentiation of osteoclasts. Osteoclasts are recruited on the surface of trabeculae, and are exposed to fluid flow caused by the deformation of the bone matrix. However, the roles of fluid shear stress (FSS) on calcium response during the differentiation process of osteoclasts are still unknown. In the current study, the formation of tartrate-resistant acid phosphatase-positive, multinucleated osteoclasts from RAW264.7 macrophage cells were induced by co-culturing them with the conditioned medium from MC3T3-E1 osteoblasts. The in situ observations showed a high correlation between the area and the nuclear number of osteoclasts. The cells were stimulated by FSS at different levels (1 or 10 dyne/cm(2)) before (0 day) or after being induced for 4 or 8 days. The mechanically-induced calcium response was recorded and analyzed. The results indicated a different property of calcium oscillation for the osteoclasts in different fusion stages (i.e., more calcium-responsive peaks appeared in small osteoclasts than those in the larger ones). The rates of calcium influx decreased and the time of recovery in osteoclast cytosol increased along with the fusion of osteoclasts. In addition, increasing the FSS level enhanced the calcium oscillation of osteoclasts at early induction (4 days). However, this effect was weakened at the late induction (8 days). The present work could help provide understanding regarding the mechanism of the involvement of calcium in mechanically induced bone remodeling.
Keyword: Osteoclasts ; Fluid shear stress ; Calcium oscillation ; Induction time ; Differentiation ; Mechanical Strain ; Rat Osteoclasts ; Bone-Resorption ; Activation ; Expression ; Cells ; Ca2+
Language: 英语
Indexed Type: SCI ; EI
Corresponding Author: Huo, B ; Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, 15 N 4th Ring Rd, Beijing 100190, Peoples R China.
Correspondent Email: huobo@imech.ac.cn;dingz@china.com.cn
Related URLs: 查看原文
WOS ID: WOS:000307400900003
ISSN: 0090-6964
Rank: [Hu, Man;Sun, Shujin; Zhang, Yan; Gao, Yuxin; Long, Mian; Huo, Bo] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, Beijing 100190, Peoples R China; [Li, Ping; Zhang, Ding] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Fac Surg, Dept Stomatol, Beijing 100730, Peoples R China
Subject: 交叉与边缘领域的力学::生物力学
Department: NML分子-细胞生物力学与空间生命科学
Classification: 二类/Q2
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/46584
Appears in Collections:国家微重力实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
SCI-J2012-082.pdf(887KB)----开放获取--View Download

Recommended Citation:
Li P,Hu M,Sun SJ,et al. Fluid flow-induced calcium response in early or late differentiated osteoclasts[J]. ANNALS OF BIOMEDICAL ENGINEERING,2012-09-01,40(9):1874-1883.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li P(李平)]'s Articles
[Hu M(胡漫)]'s Articles
[Sun SJ(孙树津)]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li P(李平)]‘s Articles
[Hu M(胡漫)]‘s Articles
[Sun SJ(孙树津)]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: SCI-J2012-082.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院力学研究所 - Feedback
Powered by CSpace