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Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry
Huang CH; Hou CJ; Huo DQ; Chen YY; Jin G(靳刚); Huo, DQ (reprint author), Chongqing Univ, Bioengn Coll, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400030, Peoples R China.
Source PublicationINTEGRATED FERROELECTRICS
2013
Volume145Issue:1Pages:122-129
ISSN1058-4587
AbstractThis study describes a simple and rapid method for virus detection on nanoscale measurement utilizing biosensor based on imaging ellipsometry. Porcine reproductive and respiratory syndrome virus (PRRSV) was served as an experimental model for demonstration. Staphylococcal protein A was firstly attached onto carboxyl-modified silicon substrate and purified anti-PRRSV IgG obtained from hyperimmune sera combined with Protein A for oriented immobilization to form capture layer. PRRSV in samples can then be specifically grabbed onto the capture layer. The signal (grayscale value) after virus combination is intuitively quantified by imaging ellipsometry. The minimum detection limit was as low as 2.4 x 10(3) median cell infectious dose (CCID50 mL(-1)) infectious virus. Application the proposed method to 20 clinic samples demonstrated that the result was significant correlated (R = 0.85) with virus isolation. The detection time was only 20min expended.
KeywordBiosensor Imaging Ellipsometry Prrsv
Subject Area交叉与边缘领域的力学
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Indexed BySCI ; EI
Language英语
WOS IDWOS:000321687200016
Funding Organizationlaboratory of Sichuan, China [NJ2009-04]
DepartmentNML纳米生物光学
ClassificationQ4
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/47361
Collection国家微重力实验室
Corresponding AuthorHuo, DQ (reprint author), Chongqing Univ, Bioengn Coll, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400030, Peoples R China.
Recommended Citation
GB/T 7714
Huang CH,Hou CJ,Huo DQ,et al. Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry[J]. INTEGRATED FERROELECTRICS,2013,145(1):122-129.
APA Huang CH,Hou CJ,Huo DQ,Chen YY,Jin G,&Huo, DQ .(2013).Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry.INTEGRATED FERROELECTRICS,145(1),122-129.
MLA Huang CH,et al."Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry".INTEGRATED FERROELECTRICS 145.1(2013):122-129.
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