Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry

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	Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry
	Huang CH; Hou CJ; Huo DQ; Chen YY; Jin G(靳刚); Huo, DQ (reprint author), Chongqing Univ, Bioengn Coll, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400030, Peoples R China.
发表期刊	INTEGRATED FERROELECTRICS
	2013
卷号	145 期号:1 页码:122-129
ISSN	1058-4587
摘要	This study describes a simple and rapid method for virus detection on nanoscale measurement utilizing biosensor based on imaging ellipsometry. Porcine reproductive and respiratory syndrome virus (PRRSV) was served as an experimental model for demonstration. Staphylococcal protein A was firstly attached onto carboxyl-modified silicon substrate and purified anti-PRRSV IgG obtained from hyperimmune sera combined with Protein A for oriented immobilization to form capture layer. PRRSV in samples can then be specifically grabbed onto the capture layer. The signal (grayscale value) after virus combination is intuitively quantified by imaging ellipsometry. The minimum detection limit was as low as 2.4 x 10(3) median cell infectious dose (CCID50 mL(-1)) infectious virus. Application the proposed method to 20 clinic samples demonstrated that the result was significant correlated (R = 0.85) with virus isolation. The detection time was only 20min expended.
关键词	Biosensor Imaging Ellipsometry Prrsv
学科领域	交叉与边缘领域的力学
URL	查看原文
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000321687200016
项目资助者	laboratory of Sichuan, China [NJ2009-04]
课题组名称	NML纳米生物光学
论文分区	Q4
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/47361
专题	微重力重点实验室
通讯作者	Huo, DQ (reprint author), Chongqing Univ, Bioengn Coll, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400030, Peoples R China.
推荐引用方式 GB/T 7714	Huang CH,Hou CJ,Huo DQ,et al. Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry[J]. INTEGRATED FERROELECTRICS,2013,145,1,:122-129.
APA	Huang CH,Hou CJ,Huo DQ,Chen YY,Jin G,&Huo, DQ .(2013).Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry.INTEGRATED FERROELECTRICS,145(1),122-129.
MLA	Huang CH,et al."Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry".INTEGRATED FERROELECTRICS 145.1(2013):122-129.

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