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Differential regulation of stiffness, topography, and dimension of substrates in rat mesenchymal stem cells
Li Z(李展); Gong YW(宫元卫); Sun SJ(孙树津); Du Y(杜宇); Lv DY(吕东媛); Liu XF(刘晓锋); Long M(龙勉); Sun, SJ (reprint author), Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China.
2013-10
发表期刊BIOMATERIALS
卷号34期号:31页码:7616-7625
ISSN0142-9612
摘要The physiological microenvironment of the stem cell niche, including the three factors of stiffness, topography, and dimension, is crucial to stem cell proliferation and differentiation. Although a growing body of evidence is present to elucidate the importance of these factors individually, the interaction of the biophysical parameters of the factors remains insufficiently characterized, particularly for stem cells. To address this issue fully, we applied a micro-fabricated polyacrylamide hydrogel substrate with two elasticities, two topographies, and three dimensions to systematically test proliferation, morphology and spreading, differentiation, and cytoskeletal re-organization of rat bone marrow mesenchymal stem cells (rBMSCs) on twelve cases. An isolated but not combinatory impact of the factors was found regarding the specific functions. Substrate stiffness or dimension is predominant in regulating cell proliferation by fostering cell growth on stiff, unevenly dimensioned substrate. Topography is a key factor for manipulating cell morphology and spreading via the formation of a large spherical shape in a pillar substrate but not in a grooved substrate. Although stiffness leads to osteogenic or neuronal differentiation of rBMSCs on a stiff or soft substrate, respectively, topography or dimension also plays a lesser role in directing cell differentiation. Neither an isolated effect nor a combinatory effect was found for actin or tubulin expression, whereas a seemingly combinatory effect of topography and dimension was found in manipulating vimentin expression. These results further the understandings of stem cell proliferation, morphology, and differentiation in a physiologically mimicking microenvironment.
关键词Stiffness Topography Dimension Stem Cell Proliferation Differentiation
学科领域生物力学
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收录类别SCI ; EI
语种英语
WOS记录号WOS:000323459100006
项目资助者National Natural Science Foundation of China [31110103918]; National Key Basic Research Foundation of China [2011CB710904]; Strategic Priority Research Program [XDA01030102]; National High Technology Research and Development Program of China [2011AA020109]
课题组名称NML分子-细胞生物力学与空间生命科学
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被引频次:63[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://dspace.imech.ac.cn/handle/311007/47445
专题国家微重力实验室
通讯作者Sun, SJ (reprint author), Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China.
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GB/T 7714
Li Z,Gong YW,Sun SJ,et al. Differential regulation of stiffness, topography, and dimension of substrates in rat mesenchymal stem cells[J]. BIOMATERIALS,2013,34(31):7616-7625.
APA Li Z.,Gong YW.,Sun SJ.,Du Y.,Lv DY.,...&Sun, SJ .(2013).Differential regulation of stiffness, topography, and dimension of substrates in rat mesenchymal stem cells.BIOMATERIALS,34(31),7616-7625.
MLA Li Z,et al."Differential regulation of stiffness, topography, and dimension of substrates in rat mesenchymal stem cells".BIOMATERIALS 34.31(2013):7616-7625.
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