中国科学院力学研究所机构知识库
Advanced  
IMECH-IR  > 国家微重力实验室  > 期刊论文
Title: Differential regulation of stiffness, topography, and dimension of substrates in rat mesenchymal stem cells
Author: Li Z(李展); Gong YW(宫元卫); Sun SJ(孙树津); Du Y(杜宇); Lv DY(吕东媛); Liu XF(刘晓锋); Long M(龙勉)
Source: BIOMATERIALS
Issued Date: 2013-10
Volume: 34, Issue:31, Pages:7616-7625
Abstract: The physiological microenvironment of the stem cell niche, including the three factors of stiffness, topography, and dimension, is crucial to stem cell proliferation and differentiation. Although a growing body of evidence is present to elucidate the importance of these factors individually, the interaction of the biophysical parameters of the factors remains insufficiently characterized, particularly for stem cells. To address this issue fully, we applied a micro-fabricated polyacrylamide hydrogel substrate with two elasticities, two topographies, and three dimensions to systematically test proliferation, morphology and spreading, differentiation, and cytoskeletal re-organization of rat bone marrow mesenchymal stem cells (rBMSCs) on twelve cases. An isolated but not combinatory impact of the factors was found regarding the specific functions. Substrate stiffness or dimension is predominant in regulating cell proliferation by fostering cell growth on stiff, unevenly dimensioned substrate. Topography is a key factor for manipulating cell morphology and spreading via the formation of a large spherical shape in a pillar substrate but not in a grooved substrate. Although stiffness leads to osteogenic or neuronal differentiation of rBMSCs on a stiff or soft substrate, respectively, topography or dimension also plays a lesser role in directing cell differentiation. Neither an isolated effect nor a combinatory effect was found for actin or tubulin expression, whereas a seemingly combinatory effect of topography and dimension was found in manipulating vimentin expression. These results further the understandings of stem cell proliferation, morphology, and differentiation in a physiologically mimicking microenvironment.
Keyword: Stiffness ; Topography ; Dimension ; Stem cell ; Proliferation ; Differentiation
Language: 英语
Indexed Type: SCI ; EI
Corresponding Author: Sun, SJ (reprint author), Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China.
Correspondent Email: sunshj@imech.ac.cn; mlong@imech.ac.cn
Related URLs: 查看原文
WOS ID: WOS:000323459100006
ISSN: 0142-9612
Rank: [Li, Zhan;Gong, Yuanwei;Sun, Shujin;Du, Yu;Lu, Dongyuan;Liu, Xiaofeng;Long, Mian] Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China; Chinese Acad Sci, Key Lab Micrograv, Nat Micrograv Lab, Inst Mech, Beijing 100190, Peoples R China
Subject: 交叉与边缘领域的力学::生物力学
Department: NML分子-细胞生物力学与空间生命科学
Classification: 一类
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/47445
Appears in Collections:国家微重力实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
IMCAS-J2013-195.pdf(1994KB)----开放获取--View Download

Recommended Citation:
李展;宫元卫;孙树津;杜宇;吕东媛;刘晓锋;龙勉.Differential regulation of stiffness, topography, and dimension of substrates in rat mesenchymal stem cells,BIOMATERIALS,2013,34(31):7616-7625
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li Z(李展)]'s Articles
[Gong YW(宫元卫)]'s Articles
[Sun SJ(孙树津)]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li Z(李展)]‘s Articles
[Gong YW(宫元卫)]‘s Articles
[Sun SJ(孙树津)]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: IMCAS-J2013-195.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院力学研究所 - Feedback
Powered by CSpace