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Asymmetric Migration of Human Keratinocytes under Mechanical Stretch and Cocultured Fibroblasts in a Wound Repair Model
Lü DY(吕东媛); Liu XF(刘晓锋); Gao YX(高宇欣); Huo B(霍波); Kang YY(康英永); Chen J(陈娟); Sun SJ(孙树津); Chen L; Luo XD; Long M(龙勉); Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100080, Peoples R China
Source PublicationPLOS ONE
2013-09-23
Volume8Issue:9Pages:e74563/1-e74563/13
ISSN1932-6203
AbstractKeratinocyte migration during re-epithelization is crucial in wound healing under biochemical and biomechanical microenvironment. However, little is known about the underlying mechanisms whereby mechanical tension and cocultured fibroblasts or keratinocytes modulate the migration of keratinocytes or fibroblasts. Here we applied a tensile device together with a modified transwell assay to determine the lateral and transmembrane migration dynamics of human HaCaT keratinocytes or HF fibroblasts. A novel pattern of asymmetric migration was observed for keratinocytes when they were cocultured with non-contact fibroblasts, i.e., the accumulative distance of HaCaT cells was significantly higher when moving away from HF cells or migrating from down to up cross the membrane than that when moving close to HF cells or when migrating from up to down, whereas HF migration was symmetric. This asymmetric migration was mainly regulated by EGF derived from fibroblasts, but not transforming growth factor alpha or beta_1 production. Mechanical stretch subjected to fibroblasts fostered keratinocyte asymmetric migration by increasing EGF secretion, while no role of mechanical stretch was found for EGF secretion by keratinocytes. These results provided a new insight into understanding the regulating mechanisms of two or three-dimensional migration of keratinocytes or fibroblasts along or across dermis and epidermis under biomechanical microenvironment.
Subject Area生物力学
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Indexed BySCI
Language英语
WOS IDWOS:000326520200036
Funding OrganizationThis work was supported by National Natural Science Foundation of China grants 30730032, 30730093, 31000421, and 30870601 (http://www.nsfc.gov.cn/Portal0/default106.htm); National Key Basic Research Foundation of China grant 2011CB710904, and National High Technology Research and Development Program of China grant 2011AA020109 (http://www.most.gov.cn/eng/index.htm), and Chinese Academy of Sciences Knowledge Innovation Program grant KJCX2-YW-L08 and Scientific Research Equipment Project of grant Y2010030 (http://english.cas.cn/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
DepartmentNML分子-细胞生物力学与空间生命科学
Classification二类/Q1
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/47498
Collection国家微重力实验室
Corresponding AuthorLong, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Lü DY,Liu XF,Gao YX,et al. Asymmetric Migration of Human Keratinocytes under Mechanical Stretch and Cocultured Fibroblasts in a Wound Repair Model[J]. PLOS ONE,2013,8(9):e74563/1-e74563/13.
APA Lü DY.,Liu XF.,Gao YX.,Huo B.,Kang YY.,...&Long, MA .(2013).Asymmetric Migration of Human Keratinocytes under Mechanical Stretch and Cocultured Fibroblasts in a Wound Repair Model.PLOS ONE,8(9),e74563/1-e74563/13.
MLA Lü DY,et al."Asymmetric Migration of Human Keratinocytes under Mechanical Stretch and Cocultured Fibroblasts in a Wound Repair Model".PLOS ONE 8.9(2013):e74563/1-e74563/13.
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