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Spreading area and shape regulate apoptosis and differentiation of osteoblasts
Fu RR(付瑞荣); Liu QL(刘琴丽); Song GB; Baik A; Hu M(胡漫); Sun SJ(孙树津); Guo XE; Long M(龙勉); Huo B(霍波); Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China.
Source PublicationBIOMEDICAL MATERIALS
2013-10
Volume8Issue:5Pages:055005/1-055005/8
ISSN1748-6041
AbstractThe in vivo observations have indicated that at the remodeling sites of bone, the spreading area or shape of preosteoblasts is confined by the mineralized matrix. But it remains unknown whether this spreading confinement regulates the differentiation or apoptosis of osteoblasts. In the present study, osteoblast-like cells (MC3T3-E1) were seeded on micropatterned islands with different area and shape. The expression of three osteogenic differentiation markers was measured by immunofluorescence staining and apoptotic cells were detected using a terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labelling assay kit. The membrane fluorescence staining results showed that the actual spreading area of micropatterned osteoblasts coincided with the designed value. When the area of a micropatterned cell was confined as 314 or 615 mu m(2), which was lower than that of freely spreading osteoblasts, the circular shape promoted the expression of osteogenic differentiation markers and the percentage of apoptotic osteoblasts compared with the branched shape. This shape-regulated differentiation and apoptosis of osteoblasts with confined spreading area were abolished when actin polymerization was inhibited by cytochalasin D. The present study gives an insight into the roles of spreading morphology on osteoblastic differentiation and apoptosis.
Subject Area生物力学
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Indexed BySCI ; EI
Language英语
WOS IDWOS:000324922200005
Funding OrganizationNational Natural Science Foundation of China [30970707, 31110103918]; National Key Basic Research Foundation of China [2011CB710904]
DepartmentNML分子-细胞生物力学与空间生命科学
Classification二类
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Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/47499
Collection国家微重力实验室
Corresponding AuthorLong, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Fu RR,Liu QL,Song GB,et al. Spreading area and shape regulate apoptosis and differentiation of osteoblasts[J]. BIOMEDICAL MATERIALS,2013,8(5):055005/1-055005/8
APA 付瑞荣.,刘琴丽.,Song GB.,Baik A.,胡漫.,...&Long, M .(2013).Spreading area and shape regulate apoptosis and differentiation of osteoblasts.BIOMEDICAL MATERIALS,8(5),055005/1-055005/8.
MLA 付瑞荣,et al."Spreading area and shape regulate apoptosis and differentiation of osteoblasts".BIOMEDICAL MATERIALS 8.5(2013):055005/1-055005/8.
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