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Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor
Liu L(刘丽); Viallat A; Jin G(靳刚); Viallat, A (reprint author), Aix Marseille Univ, CNRS, UMR 7333, INSERM,LAI,UMR 1067, F-13009 Marseille, France.
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
2014-01
Volume190Pages:221-226
ISSN0925-4005
AbstractThe behavior of giant lipid vesicles (GUVs) interacting with an adhesive surface coated with poly-L-lysine is investigated by a biosensor based on total internal reflection imaging ellipsometry (TIRIE). The adhesion of GUVs on the adhesive surface is regulated by the concentration of the poly-L-lysine coating, the pH of the vesicle suspension, and the flow rate of the suspending fluid. The TIRIE biosensor detects very clearly and sensitively GUV adsorption, flattening, rupture, and de-adhesion, all these behaviors being independently observed by phase contrast microscopy. These results show that the setting of the TIRIE biosensor, optimized for molecular sensing can be extended to GUV sensing. This work paves the way to develop a new sensitive high-throughput, low-consumption and low-cost biosensor technology for micron-size objects such as cells, capsules and liposomes.
KeywordGiant Lipid Vesicles (Guvs) Vesicles Adhesion Non-specific Adhesion Total Internal Reflection Imaging Ellipsometry (Tirie) Biosensor
Subject Area交叉与边缘领域的力学
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Indexed BySCI ; EI
Language英语
WOS IDWOS:000326687700029
DepartmentNML纳米生物光学
Classification二类/Q1
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/48076
Collection国家微重力实验室
Corresponding AuthorViallat, A (reprint author), Aix Marseille Univ, CNRS, UMR 7333, INSERM,LAI,UMR 1067, F-13009 Marseille, France.
Recommended Citation
GB/T 7714
Liu L,Viallat A,Jin G,et al. Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,190:221-226
APA 刘丽,Viallat A,靳刚,&Viallat, A .(2014).Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor.SENSORS AND ACTUATORS B-CHEMICAL,190,221-226.
MLA 刘丽,et al."Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor".SENSORS AND ACTUATORS B-CHEMICAL 190(2014):221-226.
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