| Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor |
| Liu L(刘丽) ; Viallat A; Jin G(靳刚); Viallat, A (reprint author), Aix Marseille Univ, CNRS, UMR 7333, INSERM,LAI,UMR 1067, F-13009 Marseille, France.
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| 发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL
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| 2014-01
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| 卷号 | 190页码:221-226 |
| ISSN | 0925-4005
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| 摘要 | The behavior of giant lipid vesicles (GUVs) interacting with an adhesive surface coated with poly-L-lysine is investigated by a biosensor based on total internal reflection imaging ellipsometry (TIRIE). The adhesion of GUVs on the adhesive surface is regulated by the concentration of the poly-L-lysine coating, the pH of the vesicle suspension, and the flow rate of the suspending fluid. The TIRIE biosensor detects very clearly and sensitively GUV adsorption, flattening, rupture, and de-adhesion, all these behaviors being independently observed by phase contrast microscopy. These results show that the setting of the TIRIE biosensor, optimized for molecular sensing can be extended to GUV sensing. This work paves the way to develop a new sensitive high-throughput, low-consumption and low-cost biosensor technology for micron-size objects such as cells, capsules and liposomes. |
| 关键词 | Giant Lipid Vesicles (Guvs)
Vesicles Adhesion
Non-specific Adhesion
Total Internal Reflection Imaging
Ellipsometry (Tirie)
Biosensor
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| 学科领域 | 交叉与边缘领域的力学
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| URL | 查看原文
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| 收录类别 | SCI
; EI
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| 语种 | 英语
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| WOS记录号 | WOS:000326687700029
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| 课题组名称 | NML纳米生物光学
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| 论文分区 | 二类/Q1
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/48076
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| 专题 | 微重力重点实验室
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| 通讯作者 | Viallat, A (reprint author), Aix Marseille Univ, CNRS, UMR 7333, INSERM,LAI,UMR 1067, F-13009 Marseille, France. |
推荐引用方式
GB/T 7714 |
Liu L,Viallat A,Jin G,et al. Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,190:221-226.
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| APA |
刘丽,Viallat A,靳刚,&Viallat, A .(2014).Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor.SENSORS AND ACTUATORS B-CHEMICAL,190,221-226.
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| MLA |
刘丽,et al."Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor".SENSORS AND ACTUATORS B-CHEMICAL 190(2014):221-226.
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