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Distinct kinetics features of LFA-1 and Mac-1 in neutrophil activation
Li N(李宁); Mao DB(毛德斌); Gong YX(龚一心); Lv SQ(吕守芹); Zhang Y(章燕); Long M(龙勉)
Source PublicationWorld Congress on Medical Physics and Biomedical Engineering
Conference NameWorld Congress on Medical Physics and Biomedical Engineering
Conference DateMAY 26-31, 2012
Conference PlaceBeijing, China
AbstractLFA-1 and Mac-1, two β2 integrin members constitutively expressed on neutrophils, mediate leukocyte recruitment cascade by binding to the same ligand of ICAM-1. The slow rolling and firm adhesion of leukocytes rely on LFA-1 while the cell crawling is dependent on Mac-1. We hypothesized that their distinct roles are likely attributed to the differences in the binding kinetics or in the diverse responses of outside-in and inside-out signaling. In this study, we compared the ICAM-1 binding features between soluble or membrane-expressed LFA-1 and Mac-1 with different affinity conformation using optical trap technique. Our data indicated that the affinity up-regulation from wide type (WT) to high affinity (HA) is off-rate dependent for LFA-1 but on-rate dependent for Mac-1. The structural bases of this new finding were found to be consistent with our previous simulations. These results furthered our understanding in their function differences under shear flow.
KeywordLfa-1 Mac-1 Icam-1 On-rate Off-rate
Indexed ByEI
Document Type会议论文
Corresponding AuthorLong M(龙勉)
Recommended Citation
GB/T 7714
Li N,Mao DB,Gong YX,et al. Distinct kinetics features of LFA-1 and Mac-1 in neutrophil activation[C]World Congress on Medical Physics and Biomedical Engineering,2013:139-142.
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