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A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts
Yu, WL; Qu, H; Hu GQ(胡国庆); Zhang, Q; Song, K; Guan, HJ; Liu, TJ; Qin, JH; Liu, TJ (reprint author), Dalian Med Univ, Coll Stomatol, Sect Oral Pathol, Dalian, Peoples R China.
Source PublicationPLOS ONE
2014-02-27
Volume9Issue:2Pages:e89966
ISSN1932-6203
AbstractInterstitial fluid flow (IFF) within the extracellular matrix (ECM) produces low magnitude shear stresses on cells. Fluid flow-induced stress (FSS) plays an important role during tissue morphogenesis. To investigate the effect of low FSS generated by IFF on cells, we developed a microfluidic-based cell culture device that can generate multiple low shear stresses. By changing the length and width of the flow-in channels, different continuous low level shear stresses could be generated in individual cell culture chambers. Numerical calculations demonstrate uniform shear stress distributions of the major cell culture area of each chamber. This calculation is further confirmed by the wall shear stress curves. The effects of low FSS on MC3T3-E1 proliferation and differentiation were studied using this device. It was found that FSS ranging from 1.5 to 52.6 mu Pa promoted MC3T3-E1 proliferation and differentiation, but FSS over 412 mu Pa inhibited the proliferation and differentiation of MC3T3-E1 cells. FSS ranging from 1.5 to 52.6 mu Pa also increased the expression of Runx2, a key transcription factor regulating osteoblast differentiation. It is suggested that Runx2 might be an important regulator in low FSS-induced MC3T3-E1 differentiation. This device allows for detailed study of the effect of low FSS on the behaviors of cells; thus, it would be a useful tool for analysis of the effects of IFF-induced shear stresses on cells.
KeywordElasto-plastic-damage Behavior Anisotropy Physical Mechanism Finite Deformation Constitutive Theory Component Assembling Model
Subject AreaScience & Technology - Other Topics
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Indexed BySCI
Language英语
WOS IDWOS:000332390800061
Funding OrganizationThis work was supported by National Natural Science Foundation of China (No. 81171425) and Program for Liaoning Excellent Talents in University (No. LR201013). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
DepartmentLNM微纳米流体力学
Classification二类/Q1
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/48765
Collection非线性力学国家重点实验室
Corresponding AuthorLiu, TJ (reprint author), Dalian Med Univ, Coll Stomatol, Sect Oral Pathol, Dalian, Peoples R China.
Recommended Citation
GB/T 7714
Yu, WL,Qu, H,Hu GQ,et al. A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts[J]. PLOS ONE,2014,9(2):e89966.
APA Yu, WL.,Qu, H.,Hu GQ.,Zhang, Q.,Song, K.,...&Liu, TJ .(2014).A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts.PLOS ONE,9(2),e89966.
MLA Yu, WL,et al."A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts".PLOS ONE 9.2(2014):e89966.
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