| Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading | |
Li JJ; Han D; Zhao YP(赵亚溥) ; Zhao, YP (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China.
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| Source Publication | Scientific Reports
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| 2014-01-28 | |
| Volume | 4Pages:3910 |
| ISSN | 2045-2322 |
| Abstract | To describe detailed behaviour of cell spreading under the influence of substrate stiffness, A549 cells cultured on the surfaces of polydimethylsiloxane (PDMS) and polyacrylamide (PAAm) with bulk rigidities ranging from 0.1 kPa to 40 kPa were in situ observed. The spreading behaviour of cells on PAAm presented a positive correlation between spreading speed and substrate stiffness. After computing the deformations of PAAm gels and collagen, the bulk stiffness of PAAm, rather than matrix tethering, determined the cell behaviour. On the other hand, spreading behaviour of the cells was unaffected by varying the bulk stiffness of PDMS. Based on simulation analyses, the elasticity of silica-like layer induced by UV radiation on PDMS surface dominated cell-substrate interaction, rather than the bulk stiffness of the material, indicating that it is the interfacial stiffness that mainly guided the cell spreading. And then the kinetics of cell spreading was for the first time modeled based on absolute rate theory. |
| Subject Area | Science & Technology - Other Topics |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000331152700004 |
| Funding Organization | This work was jointly supported by the National Natural Science Foundation of China (NSFC, Grant No. 11072244), the Key Research Program of the Chinese Academy of Sciences (Grant No. KJZD-EW-M01), the Instrument Developing Project of the Chinese Academy of Sciences (Grant No. Y2010031) and the project of 973 in Ministry of Science and Technology of China (Grant No. 2012CB933800). |
| Department | LNM纳/微系统力学与物理力学 |
| Classification | 一类 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/48779 |
| Collection | 非线性力学国家重点实验室 |
| Corresponding Author | Zhao, YP (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China. |
| Recommended Citation GB/T 7714 | Li JJ,Han D,Zhao YP,et al. Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading[J]. Scientific Reports,2014,4:3910. |
| APA | Li JJ,Han D,Zhao YP,&Zhao, YP .(2014).Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading.Scientific Reports,4,3910. |
| MLA | Li JJ,et al."Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading".Scientific Reports 4(2014):3910. |
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