| Differential regulation of morphology and stemness of mouse embryonic stem cells by substrate stiffness and topography | |
Lv DY(吕东媛); Luo CH; Zhang C ; Li Z; Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China.
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| 发表期刊 | BIOMATERIALS
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| 2014-04 | |
| 卷号 | 35期号:13页码:3945-3955 |
| ISSN | 0142-9612 |
| 摘要 | The maintenance of stem cell pluripotency or sternness is crucial to embryonic development and differentiation. The mechanical or physical microenvironment of stem cells, which includes extracellular matrix stiffness and topography, regulates cell morphology and stemness. Although a growing body of evidence has shown the importance of these factors in stem cell differentiation, the impact of these biophysical or biomechanical regulators remains insufficiently characterized. In the present study, we applied a micro-fabricated polyacrylamide hydrogel substrate with two elasticities and three topographies to systematically test the morphology, proliferation, and sternness of mESCs. The independent or combined impact of the two factors on specific cell functions was analyzed. Cells are able to grow effectively on both polystyrene and polyacrylamide substrates in the absence of feeder cells. Substrate stiffness is predominant in preserving stemness by enhancing Oct-4 and Nanog expression on a soft polyacrylamide substrate. Topography is also a critical factor for manipulating sternness via the formation of a relatively flattened colony on a groove or pillar substrate and a spheroid colony on a hexagonal substrate. Although topography is less effective on soft substrates, it plays a role in retaining cell sternness on stiff, hexagonal or pillar-shaped substrates. mESCs also form, in a timely manner, a 3D structure on groove or hexagonal substrates. These results further the understanding of stem cell morphology and stemness in a microenvironment that mimics physiological conditions. (C) 2014 Elsevier Ltd. All rights reserved. |
| 关键词 | Stiffness Topography Stem Cell Sternness Morphology |
| 学科领域 | Engineering ; Materials Science |
| URL | 查看原文 |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334481000004 |
| 项目资助者 | This work was supported by National Key Basic Research Foundation of China grant 2011CB710904, National Natural Science Foundation of China grant 31110103918 and 31000421, Strategic Priority Research Program grant XDA01030102, National High Technology Research and Development Program of China grant 2011AA020109. |
| 课题组名称 | NML分子-细胞生物力学与空间生命科学 |
| 论文分区 | 一类 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://dspace.imech.ac.cn/handle/311007/48843 |
| 专题 | 微重力重点实验室 |
| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Lv DY,Luo CH,Zhang C,et al. Differential regulation of morphology and stemness of mouse embryonic stem cells by substrate stiffness and topography[J]. BIOMATERIALS,2014,35,13,:3945-3955. |
| APA | Lv DY,Luo CH,Zhang C,Li Z,Long M,&Long, M .(2014).Differential regulation of morphology and stemness of mouse embryonic stem cells by substrate stiffness and topography.BIOMATERIALS,35(13),3945-3955. |
| MLA | Lv DY,et al."Differential regulation of morphology and stemness of mouse embryonic stem cells by substrate stiffness and topography".BIOMATERIALS 35.13(2014):3945-3955. |
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