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Title:
Differential regulation of morphology and stemness of mouse embryonic stem cells by substrate stiffness and topography
Author: Lv DY(吕东媛); Luo CH; Zhang C; Li Z; Long M(龙勉)
Source: BIOMATERIALS
Issued Date: 2014-04
Volume: 35, Issue:13, Pages:3945-3955
Abstract: The maintenance of stem cell pluripotency or sternness is crucial to embryonic development and differentiation. The mechanical or physical microenvironment of stem cells, which includes extracellular matrix stiffness and topography, regulates cell morphology and stemness. Although a growing body of evidence has shown the importance of these factors in stem cell differentiation, the impact of these biophysical or biomechanical regulators remains insufficiently characterized. In the present study, we applied a micro-fabricated polyacrylamide hydrogel substrate with two elasticities and three topographies to systematically test the morphology, proliferation, and sternness of mESCs. The independent or combined impact of the two factors on specific cell functions was analyzed. Cells are able to grow effectively on both polystyrene and polyacrylamide substrates in the absence of feeder cells. Substrate stiffness is predominant in preserving stemness by enhancing Oct-4 and Nanog expression on a soft polyacrylamide substrate. Topography is also a critical factor for manipulating sternness via the formation of a relatively flattened colony on a groove or pillar substrate and a spheroid colony on a hexagonal substrate. Although topography is less effective on soft substrates, it plays a role in retaining cell sternness on stiff, hexagonal or pillar-shaped substrates. mESCs also form, in a timely manner, a 3D structure on groove or hexagonal substrates. These results further the understanding of stem cell morphology and stemness in a microenvironment that mimics physiological conditions. (C) 2014 Elsevier Ltd. All rights reserved.
Keyword: Stiffness ; Topography ; Stem cell ; Sternness ; Morphology
Language: 英语
Indexed Type: SCI ; EI
Corresponding Author: Long, M (reprint author), Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China.
Correspondent Email: mlong@imech.ac.cn
Related URLs: 查看原文
WOS ID: WOS:000334481000004
ISSN: 0142-9612
Rank: [Long, Mian] Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China; Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China
Subject: Engineering; Materials Science
Department: NML分子-细胞生物力学与空间生命科学
Classification: 一类
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/48843
Appears in Collections:国家微重力实验室_期刊论文

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Recommended Citation:
Lv DY,Luo CH,Zhang C,et al. Differential regulation of morphology and stemness of mouse embryonic stem cells by substrate stiffness and topography[J]. BIOMATERIALS,2014-04-01,35(13):3945-3955.
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