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Fluid Flow-Induced Calcium Response in Osteoclasts: Signaling Pathways
Li, Ping1,2,3,4,5; Liu, Chenglin1; Hu, Man3,4; Long, Mian3,4; Zhang, Ding2; Huo, Bo1,3,4; Zhang, D (reprint author), Chinese Acad Med Sci, Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Stomatol,Fac Surg, Beijing 100730, Peoples R China.
Source PublicationANNALS OF BIOMEDICAL ENGINEERING
2014-06-01
Volume42Issue:6Pages:1250-1260
ISSN0090-6964
AbstractIntracellular calcium oscillation and its downstream signaling in osteoclasts is believed to play critical roles in regulating bone resorption. Our previous study demonstrated that fluid shear stress (FSS) induced more calcium responsive peaks in the late differentiated osteoclasts than the early ones. In this paper, the signaling pathways of FSS-induced calcium response for the osteoclasts in different differentiation stages were studied. RAW264.7 macrophage cells were induced to differentiate into osteoclasts with the conditioned medium from MC3T3-E1 osteoblasts. Furthermore pharmacological agents were added to block the specific signaling pathways. Finally the cells were exposed to FSS at different levels (1 or 10 dyne/cm(2)) after being induced for 4 or 8 days. The results showed that the mechanosensitive, cation-selective channels, phospholipase C (PLC) and endoplasmic reticulum constituted the major signaling pathway for mechanical stimulation-induced calcium response in osteoclasts. Extracellular calcium or ATP involved with calcium oscillation in a FSS magnitude-dependent manner. This pathway study may help to give insight into the molecular mechanism of mechanical stimulation-regulated bone remodeling.; Intracellular calcium oscillation and its downstream signaling in osteoclasts is believed to play critical roles in regulating bone resorption. Our previous study demonstrated that fluid shear stress (FSS) induced more calcium responsive peaks in the late differentiated osteoclasts than the early ones. In this paper, the signaling pathways of FSS-induced calcium response for the osteoclasts in different differentiation stages were studied. RAW264.7 macrophage cells were induced to differentiate into osteoclasts with the conditioned medium from MC3T3-E1 osteoblasts. Furthermore pharmacological agents were added to block the specific signaling pathways. Finally the cells were exposed to FSS at different levels (1 or 10 dyne/cm(2)) after being induced for 4 or 8 days. The results showed that the mechanosensitive, cation-selective channels, phospholipase C (PLC) and endoplasmic reticulum constituted the major signaling pathway for mechanical stimulation-induced calcium response in osteoclasts. Extracellular calcium or ATP involved with calcium oscillation in a FSS magnitude-dependent manner. This pathway study may help to give insight into the molecular mechanism of mechanical stimulation-regulated bone remodeling.
KeywordOsteoclasts Fluid Shear Stress Calcium Response Signaling Pathway Differentiation
DOI10.1007/s10439-014-0984-x
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000335653000011
WOS KeywordBONE-RESORPTION ; WAVE-PROPAGATION ; MEMBRANE STRETCH ; RAT OSTEOCLASTS ; DIFFERENTIATION ; ATP ; RECEPTOR ; CA2+ ; ACTIVATION ; NFATC1
WOS Research AreaEngineering
WOS SubjectEngineering, Biomedical
Funding OrganizationNational Natural Science Foundation of China(11372043 ; This work was supported by the National Natural Science Foundation of China [11372043 (BH) and 31070829 (DZ)], National Key Basic Research Foundation of China grant [2011CB710904 (ML)] and the Fundamental Research Funds for the Central Universities [GZ2013015101 (BH)]. ; National Key Basic Research Foundation of China(2011CB710904) ; Fundamental Research Funds for the Central Universities(GZ2013015101) ; 31070829)
DepartmentNML分子-细胞生物力学与空间生命科学
Classification二类/Q2
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/48869
Collection国家微重力实验室
Corresponding AuthorZhang, D (reprint author), Chinese Acad Med Sci, Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Stomatol,Fac Surg, Beijing 100730, Peoples R China.
Affiliation1.Beijing Inst Technol, Sch Aerosp Engn, Dept Mech, Beijing 100081, Peoples R China
2.Chinese Acad Med Sci, Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Stomatol,Fac Surg, Beijing 100730, Peoples R China
3.Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
4.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China
5.Tianjin Med Univ, Canc Inst & Hosp, Dept Maxillofacial & ENT Oncol, Tianjin 300060, Peoples R China
Recommended Citation
GB/T 7714
Li, Ping,Liu, Chenglin,Hu, Man,et al. Fluid Flow-Induced Calcium Response in Osteoclasts: Signaling Pathways[J]. ANNALS OF BIOMEDICAL ENGINEERING,2014,42(6):1250-1260.
APA Li, Ping.,Liu, Chenglin.,Hu, Man.,Long, Mian.,Zhang, Ding.,...&Zhang, D .(2014).Fluid Flow-Induced Calcium Response in Osteoclasts: Signaling Pathways.ANNALS OF BIOMEDICAL ENGINEERING,42(6),1250-1260.
MLA Li, Ping,et al."Fluid Flow-Induced Calcium Response in Osteoclasts: Signaling Pathways".ANNALS OF BIOMEDICAL ENGINEERING 42.6(2014):1250-1260.
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