The impact of N-terminal phosphorylation on LHCII conformation in state transition

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	The impact of N-terminal phosphorylation on LHCII conformation in state transition
	Ding JH(丁锦鸿); Li N(李宁); Wang ML; Zhang Y(章燕); Lv SQ(吕守芹); Long M(龙勉); Lu, SQ (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Key Lab Micrograv, Beijing 100190, Peoples R China.
发表期刊	Acta Mechanica Sinica
	2014-06
卷号	30 期号:3 页码:447-456
ISSN	0567-7718
摘要	State transition is an important protection mechanism of plants for maintaining optimal efficiency through redistributing unbalanced excitation energy between photo-system II (PSII) and photosystem I (PSI). This process depends on the reversible phosphorylation/dephosphorylation of the major light-harvesting complex II (LHCII) and its bi-directional migration between PSII and PSI. But it remains unclear how phosphorylation/dephosphorylation modulates the LHCII conformation and further regulates its reversible migration. Here molecular dynamics simulations (MDS) were employed to elucidate the impact of phosphorylation on LHCII conformation. The results indicated that N-terminal phosphorylation loosened LHCII trimer with decreased hydrogen bond (H-bond) interactions and extended the distances between neighboring monomers, which stemmed from the conformational adjustment of each monomer itself. Global conformational change of LHCII monomer started from its stromal Nterminal (including the phosphorylation sites) by enhancing its interaction to lipid membrane and by adjusting the interaction network with surrounded inter-monomer and intra-monomer transmembrane helixes of B, C, and A, and finally triggered the reorientation of transmembrane helixes and transferred the conformational change to luminal side helixes and loops. These results further our understanding in molecular mechanism of LHCII migration during state transition from the phosphorylation-induced microstructural feature of LHCII.
关键词	State Transition Lhcii Phosphorylation Conformation
学科领域	Engineering ; Mechanics
URL	查看原文
收录类别	SCI ; EI ; CSCD
语种	英语
WOS记录号	WOS:000338236800019
项目资助者	The project was supported by the National Key Basic Research Foundation of China (2006CB910303 and 2011CB710904), the National Natural Science Foundation of China (11072251 and 31230027), the CAS Knowledge Innovation Program (KJCX2-YW-L08), and the Scientific Research Equipment Project (Y2010030).
课题组名称	NML分子-细胞生物力学与空间生命科学
论文分区	二类
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/49032
专题	微重力重点实验室
通讯作者	Lu, SQ (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Key Lab Micrograv, Beijing 100190, Peoples R China.
推荐引用方式 GB/T 7714	Ding JH,Li N,Wang ML,et al. The impact of N-terminal phosphorylation on LHCII conformation in state transition[J]. Acta Mechanica Sinica,2014,30,3,:447-456.
APA	Ding JH.,Li N.,Wang ML.,Zhang Y.,Lv SQ.,...&Lu, SQ .(2014).The impact of N-terminal phosphorylation on LHCII conformation in state transition.Acta Mechanica Sinica,30(3),447-456.
MLA	Ding JH,et al."The impact of N-terminal phosphorylation on LHCII conformation in state transition".Acta Mechanica Sinica 30.3(2014):447-456.

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