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Title:
Analysis of interactions between SNARE proteins using imaging ellipsometer coupled with microfluidic array
Author: Qi C(齐财); Zhang H; Liu L(刘丽); Yang YK; Kang TF(康腾飞); Hao WX(郝文欣); Jin G(靳刚); Jiang TJ
Source: SCIENTIFIC REPORTS
Issued Date: 2014-06-18
Volume: 4, Pages:5341
Abstract: The soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins are small and abundant membrane-bound proteins, whose specific interactions mediate membrane fusion during cell fusion or cellular trafficking. In this study, we report the use of a label-free method, called imaging ellipsometer to analyze the interactions among three SNAREs, namely Sec22p, Ykt6p and Sso2p. The SNAREs were immobilized on the silicon wafer and then analyzed in a pairwise mode with microfluidic array, leading us to discover the interactions between Ykt6p and Sso2p, Sec22p and Sso2p. Moreover, by using the real-time function of the imaging ellipsometer, we were able to obtain their association constants (K-A) of about 10(4) M-1. We argue that the use of imaging ellipsometer coupled with microfluidic device will deepen our understanding of the molecular mechanisms underlying membrane fusion process.
Language: 英语
Indexed Type: SCI
Corresponding Author: Jin, G (reprint author), Chinese Acad Sci, Inst Mech, 15 Beisihuan West Rd, Beijing 100190, Peoples R China.
Correspondent Email: gajin@imech.ac.cn; taijiao@moon.ibp.ac.cn
DOI: 10.1038/srep05341
Related URLs: 查看原文
WOS ID: WOS:000337499100006
ISSN: 2045-2322
Funder: Project 973 [2014CB910500] ; National Natural Science Foundation of China [31300632, 21305147] ; National Science and Technology Major Project of the Ministry of Science and Technology of China [2013ZX10004611-002] ; National Basic Research Program (Project 973) of China [2009CB320300] ; National Basic Research Program of China [2009CB320302] ; National High Technology Research Development Program of China [2008AA02Z419] ; International Science & Technology Cooperation Program of China [2012DFG31880] ; Projects of Sino-Portugal ST Cooperation ; Instrument Developing Project of the Chinese Academy of Sciences [KJCX2-YW-M04, KJCX2-YW-M03] ; Basic Scientific Research Special Foundation of Chinese Academy of Inspection and Quarantine [2010JK002] ; China Postdoctoral Science Foundation [20080440533]
Rank: [Qi, Cai; Zhang, Hong; Jiang, Taijiao] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China; [Liu, Li; Kang, Tengfei; Hao, Wenxin; Jin, Gang] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China; [Yang, Yinke] Cent S Univ, Xiang Ya Med Sch, Changsha 410083, Hunan, Peoples R China; [Qi, Cai] Chinese Acad Inspect & Quarantine, Inst Equipment Technol, Beijing 100123, Peoples R China
Subject: Science & Technology - Other Topics
Department: NML纳米生物光学
Classification: 一类
Citation statistics:
Content Type: 期刊论文
URI: http://dspace.imech.ac.cn/handle/311007/49035
Appears in Collections:国家微重力实验室_期刊论文

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Recommended Citation:
Qi C,Zhang H,Liu L,et al. Analysis of interactions between SNARE proteins using imaging ellipsometer coupled with microfluidic array[J]. SCIENTIFIC REPORTS,2014-06-18,4:5341.
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