Attachment and Detachment Processes of Individual Lysozyme Molecules on a Surface of a Monoclinic Lysozyme Crystal Studied by Fluorescent Single-Molecule Visualization

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	Attachment and Detachment Processes of Individual Lysozyme Molecules on a Surface of a Monoclinic Lysozyme Crystal Studied by Fluorescent Single-Molecule Visualization
	Dai GL(戴国亮); Zheng, L; Sazaki, G; Furukawa, Y; Sazaki, G (reprint author), Hokkaido Univ, Inst Low Temp Sci, Kita Ku, N19-W8, Sapporo, Hokkaido 0600819, Japan.
发表期刊	CRYSTAL GROWTH & DESIGN
	2014-10
卷号	14 期号:10 页码:5303-5309
ISSN	1528-7483
摘要	On a {10 (1) over bar} face of a monoclinic crystal of hen egg-white lysozyme (HEWL), we visualized the attachment and detachment processes of individual fluorescent-labeled HEWL (F-HEWL) molecules by a fluorescent single-molecule visualization technique. We measured the changes in number density of F-HEWL molecules, whose positions were not changed for longer than a certain residence time, as a function of an adsorption time. We first confirmed that under an equilibrium condition, there was an induction period (similar to 120 min) of the attachment/detachment processes, during which period the number density remained constant. After the induction period, the number density increased linearly with the adsorption time, as it was recently found on a tetragonal HEWL crystal [Dai, G. L. Cryst. Growth Des 2011, 11 (1), 88-92]. In addition, we performed similar measurements under a supersaturated condition. Then we found that supersaturation significantly enhanced the attachment process after the induction time. The attachment/detachment processes finally reached a steady state, in which the attachment rate was higher than the detachment one. Moreover, we also found that in a rare case, an F-HEWL molecule adsorbed on a step laterally moved following the advancement of a growing step.
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收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000342609300055
项目资助者	G.D. acknowledges the support by National Program on Key Basic Research Project (973 Program) (No. 2011CB710901), and the visiting professorship from Institute of Low Temperature Science, Hokkaido University. G.S. is grateful for the support by Chinese Academy of Sciences visiting professorship for senior international scientists (Grant No. 2013T2J0060). The authors thank Y. Saito and S. Kobayashi (Olympus Engineering Co., Ltd.) for their technical support of LCM-DIM. Funding is from National Program on Key Basic Research Project (973 Program) (No. 2011CB710901); visiting professorship from Institute of Low Temperature Science, Hokkaido University; Chinese Academy of Sciences visiting professorship for senior international scientists (Grant No. 2013T2J0060).
课题组名称	NML复杂流体
论文分区	一类
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/49345
专题	微重力重点实验室
通讯作者	Sazaki, G (reprint author), Hokkaido Univ, Inst Low Temp Sci, Kita Ku, N19-W8, Sapporo, Hokkaido 0600819, Japan.
推荐引用方式 GB/T 7714	Dai GL,Zheng, L,Sazaki, G,et al. Attachment and Detachment Processes of Individual Lysozyme Molecules on a Surface of a Monoclinic Lysozyme Crystal Studied by Fluorescent Single-Molecule Visualization[J]. CRYSTAL GROWTH & DESIGN,2014,14,10,:5303-5309.
APA	Dai GL,Zheng, L,Sazaki, G,Furukawa, Y,&Sazaki, G .(2014).Attachment and Detachment Processes of Individual Lysozyme Molecules on a Surface of a Monoclinic Lysozyme Crystal Studied by Fluorescent Single-Molecule Visualization.CRYSTAL GROWTH & DESIGN,14(10),5303-5309.
MLA	Dai GL,et al."Attachment and Detachment Processes of Individual Lysozyme Molecules on a Surface of a Monoclinic Lysozyme Crystal Studied by Fluorescent Single-Molecule Visualization".CRYSTAL GROWTH & DESIGN 14.10(2014):5303-5309.

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