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Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry
Kang TF(康腾飞); Niu Y(牛宇); Jin G(靳刚); Jin, G (reprint author), 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.
Source PublicationThin Solid Films
2014-11-28
Volume571Pages:463-467
ISSN0040-6090
AbstractWeak affinity interactions among biomolecules have attracted more and more attentions for they play a significant role in organisms. However, it is difficult to characterize these interactions due to the limitation of the detection approaches. The biosensor based on total internal reflection imaging ellipsometry (TIRIE) is a real-time analysis method for biomolecular interaction detection with the advantages of high throughput and sensitivity. The interaction between tris and lysozyme (LZM), a typical example of weak affinity interactions, has been detected with TIRIE as a trial. Tris is immobilized on the gold thin film substrate to form the biosensing surface and then LZM as well as its negative controls is delivered to the tris-immobilized surface. The interaction process between tris and LZM is recorded by TIRIE biosensor to forma real-time curve and its dissociation constant is deduced as 7.6 x 10(-5) M, which is in agreement with the results in the reported work by other investigators. The results indicate that TIRIE biosensor is competent for weak affinity interaction analysis. (C) 2014 Elsevier B.V. All rights reserved.
KeywordBiosensor Total Internal Reflection Imaging Ellipsometry Weak Affinity Interaction Tris Lysozyme
Subject AreaMaterials Science ; Physics
DOI10.1016/j.tsf.2014.02.098
URL查看原文
Indexed BySCI ; EI
Language英语
WOS IDWOS:000346055200022
WOS KeywordPLASMON RESONANCE BIOSENSOR ; POLARIZATION ; RECOGNITION ; AFFINITIES ; ANTIGEN
WOS Research AreaMaterials Science ; Physics
WOS SubjectMaterials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
DepartmentNML纳米生物光学
Classification二类/Q2
Citation statistics
Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/49493
Collection国家微重力实验室
Corresponding AuthorJin, G (reprint author), 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Kang TF,Niu Y,Jin G,et al. Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry[J]. Thin Solid Films,2014,571:463-467.
APA Kang TF,Niu Y,Jin G,&Jin, G .(2014).Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry.Thin Solid Films,571,463-467.
MLA Kang TF,et al."Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry".Thin Solid Films 571(2014):463-467.
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