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Kinetic analysis of the weak affinity interaction between tris and lysozyme
Kang TF(康腾飞); Hao WX(郝文欣); Niu Y(牛宇); Luo ZR(罗子人); Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2015-02-20
Volume457Issue:4Pages:659-663
ISSN0006-291X
AbstractThe biosensor based on total internal reflection imaging ellipsometry (TIRIE), regarded as an automotive real-time research approach for biomolecular interaction, is introduced to analyze the kinetic process of the weak interaction between tris and lysozyme. The experiment is performed by delivering lysozyme solution diluted to different concentrations to the biosensor substrate interface immobilized with tris. By applying pseudo-first-order interaction kinetics model, we are able to obtain the kinetic parameters from fitting experimental data. The calculated association rate constant and dissociation rate constant of tris and lysozyme interaction are in 10(-2) mol(-1) s(-1) and 10(3) s(-1) magnitude, respectively. To further improve TIRIE's ability for kinetically characterizing biomolecular interaction, a theoretical method to deduce associate rate constant before experiment is proposed. (C) 2015 Elsevier Inc. All rights reserved.
KeywordKinetic Analysis Weak Affinity Interaction Tirie Biosensor Pseudo-first-order Interaction Kinetics Association Rate Constant
Subject AreaBiochemistry & Molecular Biology ; Biophysics
DOI10.1016/j.bbrc.2015.01.044
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000350838800028
WOS KeywordREFLECTION IMAGING ELLIPSOMETRY ; BIOMOLECULAR INTERACTIONS ; PROTEIN INTERACTIONS ; BIOSENSOR ; ADSORPTION ; MODEL
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
Funding OrganizationNational Basic Research Program of China [2015CB352100] ; National Natural Science Foundation of China [21305147, 81472941] ; International Science & Technology Cooperation Program of China [2012DFG31880] ; Seventh Sino-Portugal Scientific and Technological Cooperation
DepartmentNML纳米生物光学
ClassificationQ3
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Document Type期刊论文
Identifierhttp://dspace.imech.ac.cn/handle/311007/49926
Collection国家微重力实验室
Corresponding AuthorJin, G (reprint author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Kang TF,Hao WX,Niu Y,et al. Kinetic analysis of the weak affinity interaction between tris and lysozyme[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,457(4):659-663.
APA Kang TF,Hao WX,Niu Y,Luo ZR,Jin G,&Jin, G .(2015).Kinetic analysis of the weak affinity interaction between tris and lysozyme.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,457(4),659-663.
MLA Kang TF,et al."Kinetic analysis of the weak affinity interaction between tris and lysozyme".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 457.4(2015):659-663.
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